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Literature summary extracted from

  • Leung, H.B.; Schramm, V.L.
    A mutant AMP nucleosidase. Purification, properties and in vivo turnover of the protein (1981), J. Biol. Chem., 256, 12823-12829.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.4 MgATP2- mutant AMP nucleosidase Azotobacter vinelandii

Protein Variants

EC Number Protein Variants Comment Organism
3.2.2.4 additional information treatment with nitrosoguanidine leads to mutants defective in degrading AMP Azotobacter vinelandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.4 Formycin monophosphate mutant AMP nucleosidase Azotobacter vinelandii
3.2.2.4 phosphate mutant AMP nucleosidase Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.4 0.16
-
 AMP mutant AMP nucleosidase Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.4 54000
-
 6 * 54000, gel filtration Azotobacter vinelandii
3.2.2.4 320000
-
 gel filtration Azotobacter vinelandii
3.2.2.4 330000
-
 mutant AMP nucleosidase, gel filtration Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.4 AMP + H2O Azotobacter vinelandii
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.4 Azotobacter vinelandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.4 mutant AMP nucleosidase Azotobacter vinelandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.4 0.3
-
 mutant enzyme Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.4 AMP + H2O
-
 Azotobacter vinelandii adenine + ribose 5-phosphate
-
 ?
3.2.2.4 AMP + H2O
-
 Azotobacter vinelandii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.4 hexamer 6 * 54000, gel filtration Azotobacter vinelandii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.4 0.6
-
 AMP mutant enzyme Azotobacter vinelandii
3.2.2.4 62
-
 AMP
-
 Azotobacter vinelandii