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Literature summary extracted from
- Role of specific amino acid residues in T4 endonuclease V that alter nontarget DNA binding (1997), Biochemistry, 36, 4080-4088.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.7 | expression in Escherichia coli | Tequatrovirus T4 |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.2.17 | - |
Tequatrovirus T4 |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.21.7 | K121N | decreased affinity for nontarget DNA | Tequatrovirus T4 |
| 3.1.21.7 | K86N | decreased affinity for nontarget DNA | Tequatrovirus T4 |
| 3.1.21.7 | Q15R | limited processive nicking activity | Tequatrovirus T4 |
| 3.1.21.7 | R117N | limited processive nickin activity | Tequatrovirus T4 |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.21.7 | 0.003 | - |
49-mer oligonucleotide | mutant Q15R and R117N enzymes, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
| 3.1.21.7 | 0.005 | - |
49-mer oligonucleotide | wild type enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
| 3.1.21.7 | 0.013 | - |
49-mer oligonucleotide | mutant K121N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
| 3.1.21.7 | 0.024 | - |
49-mer oligonucleotide | mutant K86N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
| 3.2.2.17 | 0.000003 | - |
DNA | mutant Q15R, R117N | Tequatrovirus T4 |  |
| 3.2.2.17 | 0.000005 | - |
DNA | wild-type | Tequatrovirus T4 | |
| 3.2.2.17 | 0.000013 | - |
DNA | mutant K121N | Tequatrovirus T4 | |
| 3.2.2.17 | 0.000024 | - |
DNA | mutant K86N | Tequatrovirus T4 | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.7 | DNA + H2O | Tequatrovirus T4 | pyrimidine dimer-specific | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? | |
| 3.1.21.7 | double-stranded plasmid DNA + H2O | Tequatrovirus T4 | supercoiled pBR322-DNA | nicked circular DNA + linearized DNA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.7 | Tequatrovirus T4 | - |
- |
- |
| 3.2.2.17 | Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.7 | to homogeneity, chromatography steps, recombinant enzymes | Tequatrovirus T4 |
| 3.2.2.17 | site-directed mutant enzymes | Tequatrovirus T4 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.7 | 12-mer oligonucleotide + H2O | double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | hydrolyzed DNA | - |
? | |
| 3.1.21.7 | 49-mer oligonucleotide + H2O | double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | hydrolysed DNA | - |
? | |
| 3.1.21.7 | DNA + H2O | pyrimidine dimer-specific | Tequatrovirus T4 | 5'-phosphoryltermini DNA + 3'-hydroxyltermini DNA | - |
? | |
| 3.1.21.7 | double-stranded plasmid DNA + H2O | supercoiled pBR322-DNA | Tequatrovirus T4 | nicked circular DNA + linearized DNA | - |
? | |
| 3.2.2.17 | DNA + H2O | beta-elimination reaction results in an alpha,beta-unsaturated aldehyde | Tequatrovirus T4 | thymine + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.7 | T4 endonuclease V | - |
Tequatrovirus T4 |
| 3.2.2.17 | denV | - |
Tequatrovirus T4 |
| 3.2.2.17 | T4 PD-DNA-glycosylase | - |
Tequatrovirus T4 |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.17 | 15 | 37 | possibility of denaturation of the substrate at temperatures above 20°C | Tequatrovirus T4 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.21.7 | 0.000333 | - |
49-mer oligonucleotide | mutant K121N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
| 3.1.21.7 | 0.00167 | - |
49-mer oligonucleotide | mutant R117N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
| 3.1.21.7 | 0.005 | - |
49-mer oligonucleotide | mutant Q15R enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
| 3.1.21.7 | 0.00667 | - |
49-mer oligonucleotide | mutant K86N enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 | |
| 3.1.21.7 | 0.0117 | - |
49-mer oligonucleotide | wild type enzyme, double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer | Tequatrovirus T4 |
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