Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Haseltine, W.A.; Gordon, L.K.; Lindan, C.P.; Grafstrom, R.H.; Shaper, N.L.; Gropssmann, L.
    Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus (1980), Nature, 285, 634-641.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.17 MgCl2 catalytic activity 7 times greater than in presence of EDTA, DNA glycosylase activity preceded by a Mg2+ dependent AP endonuclease, followed by an AP endonuclease active in the absence of Mg2+ and in presence of EDTA Micrococcus luteus
3.2.2.17 MgCl2 not stimulated by CaCl2, MnCl2, ZnCl2 Micrococcus luteus
3.2.2.17 MgCl2 unaffected by the presence of ATP Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.17 DNA + H2O Micrococcus luteus pyrimidine cyclobutane dimer formed between adjacent pyrimidines ?
-
 ?
3.2.2.17 additional information Micrococcus luteus only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.17 Micrococcus luteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.17
-
 Micrococcus luteus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.17 thymidine-dimer-containing DNA + H2O = thymidine-dimer-containing DNA with apyrimidinic site cleaves N-glycosyl bond between pyrimidine and sugar without rupture of a phosphodiester bond Micrococcus luteus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.17 DNA + H2O
-
 Micrococcus luteus thymine + ?
-
 ?
3.2.2.17 DNA + H2O pyrimidine cyclobutane dimer formed between adjacent pyrimidines Micrococcus luteus ?
-
 ?
3.2.2.17 DNA phiX174 RFI + H2O double-stranded covalently closed circular DNA molecule contains apurinic sites Micrococcus luteus thymine + ?
-
 ?
3.2.2.17 additional information only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded Micrococcus luteus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.17 dimer specific M. luteus endonuclease
-
 Micrococcus luteus
3.2.2.17 dimer-dependent endonuclease
-
 Micrococcus luteus
3.2.2.17 UV endonuclease
-
 Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.17 6.5 8
-
 Micrococcus luteus