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Literature summary extracted from

  • Radany, E.H.; Friedberg, E.C.
    A pyrimidine dimer-DNA glycosylase activity associated with the v gene product of bacteriophage T4 (1980), Nature, 286, 183-185.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.17 DNA + H2O Tequatrovirus T4 UV-irradiated DNA, containing thymine dimers ?
-
?
3.2.2.17 additional information Tequatrovirus T4 only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.17 Tequatrovirus T4
-
bacteriophage, Escherichia coli cells infected with wild-type v+
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.17 partially Tequatrovirus T4

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.17 thymidine-dimer-containing DNA + H2O = thymidine-dimer-containing DNA with apyrimidinic site hydrolysis of phosphodiester bonds Tequatrovirus T4

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.17 infected cell infected E. coli with phage T4v+ Tequatrovirus T4
-
3.2.2.17 infected cell cells infected with wild-type phage T4 Tequatrovirus T4
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.17 DNA + H2O UV-irradiated DNA, containing thymine dimers Tequatrovirus T4 thymine + ? enzymatic photoreactivation, direct photoreversal, releases free thymine from E. coli DNA ?
3.2.2.17 DNA + H2O UV-irradiated DNA, containing thymine dimers Tequatrovirus T4 ?
-
?
3.2.2.17 additional information only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded Tequatrovirus T4 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.17 denV
-
Tequatrovirus T4
3.2.2.17 PD-specific DNA glycosylase
-
Tequatrovirus T4
3.2.2.17 T4 endonuclease V
-
Tequatrovirus T4
3.2.2.17 T4 PD-DNA-glycosylase
-
Tequatrovirus T4
3.2.2.17 T4 UV endonuclease
-
Tequatrovirus T4