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Literature summary extracted from

  • Kushad, M.M.; Richardson, D.G.; Ferro, A.J.
    5'-Methylthioadenosine nucleosidase and 5-methylthioribose kinase activities and ethylene production during tomato fruit development and ripening (1985), Plant Physiol., 79, 525-529.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.100 5-Ethylthioribose weak inhibition Solanum lycopersicum
2.7.1.100 5-Isobutylthioribose 41% inhibition Solanum lycopersicum
2.7.1.100 5-Isopropylthioribose weak inhibition Solanum lycopersicum
3.2.2.16 5'-Chloroadenosine exhibits a 35-47% inhibition of enzyme activity Solanum lycopersicum
3.2.2.16 5'-Chloroformycin substrate analogue, reduces enzyme activity by 89% Solanum lycopersicum
3.2.2.16 5'-ethylthioadenosine inhibits the reaction with methylthioadenosine by about 40% Solanum lycopersicum
3.2.2.16 5'-isobutylthioadenosine inhibits the reaction with methylthioadenosine by about 40% Solanum lycopersicum
3.2.2.16 5'-Isopropylthioadenosine inhibits the reaction with methylthioadenosine by about 40% Solanum lycopersicum
3.2.2.16 aminoethoxyvinylglycine 34% inhibition Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.16 0.015
-
 5'-deoxy-5'-methylthioadenosine
-
 Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.16 5'-methylthioadenosine + H2O Solanum lycopersicum
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.100 Solanum lycopersicum
-
 Mill cv Rutgers
-
3.2.2.16 Solanum lycopersicum
-
 tomato, Mill. cv Rutgers
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.100 fruit highest activity at the breaker stage Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.100 0.0007
-
 activity in fruit at breaker stage of ripening Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.100 ATP + 5-methylthioribose
-
 Solanum lycopersicum ADP + 5-methylthioribose 1-phosphate
-
 ?
3.2.2.16 5'-methylthioadenosine + H2O
-
 Solanum lycopersicum ?
-
 ?