Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Karran, P.; Lindahl, T.
    Hypoxanthine in deoxyribonucleic acid: generation by heat-induced hydrolysis of adenine residues and release in free form by a deoxyribonucleic acid glycosylase from calf thymus (1980), Biochemistry, 19, 6005-6011.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.2.15 fully active in presence of 1-5 mM EDTA Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.15 Ca2+ higher concentrations, above 10 mM CaCl2 Bos taurus
3.2.2.15 DNA markedly inhibited by low concentrations of native DNA Bos taurus
3.2.2.15 KCl 2fold inhibited Escherichia coli
3.2.2.15 Mg2+ higher concentrations, above 20 mM MgCl2 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.15 0.0005
-
 poly(dAdT)
-
 Bos taurus
3.2.2.15 0.0009
-
 poly-dC
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.15 KCl activity 4fold stimulated by 0.1 M KCl Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.15 30000
-
-
 Escherichia coli
3.2.2.15 31000
-
 SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.15 DNA + H2O Escherichia coli
-
 ?
-
 ?
3.2.2.15 DNA + H2O Homo sapiens
-
 ?
-
 ?
3.2.2.15 DNA + H2O Bos taurus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.15 Bos taurus
-
 calf
-
3.2.2.15 Escherichia coli
-
-
-
3.2.2.15 Homo sapiens
-
 human, normal and progeroid cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.15
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.15 skin fibroblast
-
 Homo sapiens
-
3.2.2.15 skin fibroblast
-
 Bos taurus
-
3.2.2.15 thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.15 DNA + H2O
-
 Escherichia coli hypoxanthine + ?
-
 ?
3.2.2.15 DNA + H2O preference for double-stranded substrates Bos taurus hypoxanthine + ? liberated from DNA directly as free base, direct cleavage of the base-sugar bonds of dIMP residues in the polymer ?
3.2.2.15 DNA + H2O
-
 Escherichia coli ?
-
 ?
3.2.2.15 DNA + H2O
-
 Homo sapiens ?
-
 ?
3.2.2.15 DNA + H2O
-
 Bos taurus ?
-
 ?
3.2.2.15 additional information DNA repair by initiating a base excision-repair at a damaged or misincorporated residue Escherichia coli ?
-
 ?
3.2.2.15 additional information DNA repair by initiating a base excision-repair at a damaged or misincorporated residue Homo sapiens ?
-
 ?
3.2.2.15 additional information DNA repair by initiating a base excision-repair at a damaged or misincorporated residue Bos taurus ?
-
 ?
3.2.2.15 poly(dAdT) + H2O
-
 Bos taurus ?
-
 ?
3.2.2.15 poly-dC + H2O
-
 Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.15 bovine hypoxanthine-DNA glycosylase
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.15 7.2 7.8
-
 Bos taurus