BRENDA - Enzyme Database

Arabinanase A from Pseudomonas fluorescens subsp. cellulosa exhibits both an endo- and an exo-mode of action

McKie, V.A.; Black, G.W.; Millward-Sadler, S.J.; Hazlewood, G.P.; Laurie, J.I.; Gilbert, H.J.; Biochem. J. 323, 547-555 (1997) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.99
expressed in Escherichia coli
Pseudomonas fluorescens
3.2.1.99
hyperexpression in Escherichia coli
Pseudomonas fluorescens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.99
additional information
-
additional information
-
Pseudomonas fluorescens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.99
extracellular
-
Pseudomonas fluorescens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.99
34000
-
SDS-PAGE
Pseudomonas fluorescens
3.2.1.99
39440
-
calculated from nucleotide sequence
Pseudomonas fluorescens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.99
Pseudomonas fluorescens
-
arabinanase A, exhibits endo- and exo-mode of action
-
3.2.1.99
Pseudomonas fluorescens
-
subspecies cellulosa
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.99
recombinant arabinanase
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.99
additional information
-
-
Pseudomonas fluorescens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.99
1,5-alpha-L-arabinan + H2O
linear arabinan
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
main product
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
poor substrate
171758
Pseudomonas fluorescens
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
-
-
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranose
-
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
171758
Pseudomonas fluorescens
?
3.2.1.99
carboxymethylarabinan + H2O
-
171758
Pseudomonas fluorescens
?
-
-
-
?
3.2.1.99
L-arabinan + H2O
linear arabinan
171758
Pseudomonas fluorescens
L-arabino-oligosaccharides
main product: L-arabinotriose
171758
Pseudomonas fluorescens
?
3.2.1.99
L-arabinan + H2O
branched sugar beet arabinan containing alpha-1,3-arabinose side chains: not a substrate
171758
Pseudomonas fluorescens
L-arabino-oligosaccharides
main product: L-arabinotriose
171758
Pseudomonas fluorescens
?
3.2.1.99
L-arabino-oligosaccharide + H2O
-
171758
Pseudomonas fluorescens
L-arabino-oligosaccharide
main product: L-arabinotriose
171758
Pseudomonas fluorescens
?
3.2.1.99
additional information
inactive on cellulose, avicel, amorphous cellulose, carboxymethylcellulose, xylan, galactan, mannan, 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-xyloside and 2,4-dinitrophenyl beta-xylobioside
171758
Pseudomonas fluorescens
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.99
arabinanase A
exhibits endo- and exo-mode of action
Pseudomonas fluorescens
3.2.1.99
arbA
exhibits endo- and exo-mode of action
Pseudomonas fluorescens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.99
expressed in Escherichia coli
Pseudomonas fluorescens
3.2.1.99
hyperexpression in Escherichia coli
Pseudomonas fluorescens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.99
additional information
-
additional information
-
Pseudomonas fluorescens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.99
extracellular
-
Pseudomonas fluorescens
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.99
34000
-
SDS-PAGE
Pseudomonas fluorescens
3.2.1.99
39440
-
calculated from nucleotide sequence
Pseudomonas fluorescens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.99
recombinant arabinanase
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.99
additional information
-
-
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.99
1,5-alpha-L-arabinan + H2O
linear arabinan
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
main product
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
poor substrate
171758
Pseudomonas fluorescens
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
-
-
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranose
-
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
171758
Pseudomonas fluorescens
?
3.2.1.99
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
-
171758
Pseudomonas fluorescens
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
171758
Pseudomonas fluorescens
?
3.2.1.99
carboxymethylarabinan + H2O
-
171758
Pseudomonas fluorescens
?
-
-
-
?
3.2.1.99
L-arabinan + H2O
linear arabinan
171758
Pseudomonas fluorescens
L-arabino-oligosaccharides
main product: L-arabinotriose
171758
Pseudomonas fluorescens
?
3.2.1.99
L-arabinan + H2O
branched sugar beet arabinan containing alpha-1,3-arabinose side chains: not a substrate
171758
Pseudomonas fluorescens
L-arabino-oligosaccharides
main product: L-arabinotriose
171758
Pseudomonas fluorescens
?
3.2.1.99
L-arabino-oligosaccharide + H2O
-
171758
Pseudomonas fluorescens
L-arabino-oligosaccharide
main product: L-arabinotriose
171758
Pseudomonas fluorescens
?
3.2.1.99
additional information
inactive on cellulose, avicel, amorphous cellulose, carboxymethylcellulose, xylan, galactan, mannan, 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-xyloside and 2,4-dinitrophenyl beta-xylobioside
171758
Pseudomonas fluorescens
?
-
-
-
?