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Literature summary extracted from

  • Beldman, G.; Searle-van Leeuwen, M.J.F.; De Ruiter, G.A.; Siliha, H.A.; Voragen, A.G.J.
    Degradation of arabinans by arabinanases from Aspergillus aculeatus and Aspergillus niger (1993), Carbohydr. Polym., 20, 159-168.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.99 nutrition commercial applications in fruit and vegetable processing, particularly in apple juice manufacture where their use prevents haze formation in pectinase-treated apple pulp by depolymerizing debranched arabinan, linear alpha-1,5-arabinan, formed by the action of alpha-L-arabinofuranosidases during this process Aspergillus niger
3.2.1.99 nutrition commercial applications in fruit and vegetable processing, particularly in apple juice manufacture where their use prevents haze formation in pectinase-treated apple pulp by depolymerizing debranched arabinan, linear alpha-1,5-arabinan, formed by the action of alpha-L-arabinofuranosidases during this process Aspergillus aculeatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.99 branched arabinofuranosides
-
 Aspergillus aculeatus
3.2.1.99 branched arabinofuranosides
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 45000
-
 SDS-PAGE Aspergillus niger
3.2.1.99 45000
-
 SDS-PAGE Aspergillus aculeatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Aspergillus aculeatus
-
-
-
3.2.1.99 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99
-
 Aspergillus aculeatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 1,5-alpha-L-arabinan + H2O best substrate, endo mechanism Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara action patterns of initial hydrolysis products ?
3.2.1.99 1,5-alpha-L-arabinan + H2O best substrate, endo mechanism Aspergillus aculeatus alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara action patterns of initial hydrolysis products ?
3.2.1.99 1,5-alpha-L-arabinan + H2O best substrate, endo mechanism Aspergillus niger alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara product formation, overview ?
3.2.1.99 1,5-alpha-L-arabinan + H2O best substrate, endo mechanism Aspergillus aculeatus alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara product formation, overview ?
3.2.1.99 alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O very poor substrate Aspergillus niger alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
 ?
3.2.1.99 arabinogalactan + H2O onion arabinogalactan: not a substrate Aspergillus aculeatus ?
-
 ?
3.2.1.99 arabinogalactan + H2O potato arabinogalactan Aspergillus niger ?
-
 ?
3.2.1.99 arabinogalactan + H2O potato arabinogalactan Aspergillus aculeatus ?
-
 ?
3.2.1.99 CM-cellulose + H2O poor substrate Aspergillus aculeatus ?
-
 ?
3.2.1.99 L-arabinan + H2O apple juice ultrafiltration retentate arabinan, branched sugar-beet arabinan, linear apple arabinan Aspergillus niger L-arabino-oligosaccharides end-product: low molecular weight oligomeric products ?
3.2.1.99 L-arabinan + H2O apple juice ultrafiltration retentate arabinan, branched sugar-beet arabinan, linear apple arabinan Aspergillus aculeatus L-arabino-oligosaccharides end-product: low molecular weight oligomeric products ?
3.2.1.99 L-arabinan + H2O linear and branched arabinan Aspergillus niger L-arabino-oligosaccharides end-product: low molecular weight oligomeric products ?
3.2.1.99 L-arabinan + H2O linear and branched arabinan Aspergillus aculeatus L-arabino-oligosaccharides end-product: low molecular weight oligomeric products ?
3.2.1.99 L-arabino-oligosaccharide + H2O
-
 Aspergillus niger L-arabino-oligosaccharide product formation ?
3.2.1.99 L-arabino-oligosaccharide + H2O
-
 Aspergillus aculeatus L-arabino-oligosaccharide product formation ?
3.2.1.99 L-arabino-oligosaccharide + H2O
-
 Aspergillus niger L-arabino-oligosaccharide small oligomeric products ?
3.2.1.99 L-arabino-oligosaccharide + H2O
-
 Aspergillus aculeatus L-arabino-oligosaccharide small oligomeric products ?
3.2.1.99 additional information substrate specificity, overview Aspergillus niger ?
-
 ?
3.2.1.99 additional information substrate specificity, overview Aspergillus aculeatus ?
-
 ?
3.2.1.99 additional information inactive on p-nitrophenylarabinofuranoside, onion arabinogalactan, stractan, wheat arabinoxylan, oat spelts arabinoxylan, avicel, polygalacturonic acid Aspergillus aculeatus ?
-
 ?
3.2.1.99 additional information product specificity, overview Aspergillus niger ?
-
 ?
3.2.1.99 additional information product specificity, overview Aspergillus aculeatus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 4.5 5.5
-
 Aspergillus niger
3.2.1.99 5.5
-
-
 Aspergillus aculeatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.99 5.5 6.3 stable between Aspergillus aculeatus