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Literature summary extracted from

  • Kaji, A.
    L-Arabinosidases (1984), Adv. Carbohydr. Chem. Biochem., 42, 383-394.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 32000
-
 F-11, SDS-PAGE Bacillus subtilis
3.2.1.99 33000
-
 IFO 3022, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Bacillus subtilis
-
 IFO 3022
-
3.2.1.99 Bacillus subtilis
-
 F-11
-
3.2.1.99 Bacillus subtilis F-11
-
 F-11
-
3.2.1.99 Clostridium felsineum var. sikokianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99 F-11 Bacillus subtilis
3.2.1.99 IFO 3022 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.99 7.78
-
 IFO 3022 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 1,5-alpha-L-arabinan + H2O F-11 and IFO 3022: best substrate, endo mechanism Bacillus subtilis alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose end-product ?
3.2.1.99 1,5-alpha-L-arabinan + H2O F-11 and IFO 3022: best substrate, endo mechanism Bacillus subtilis F-11 alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose end-product ?
3.2.1.99 1,5-alpha-L-arabinan + H2O F-11 and IFO 3022: best substrate, endo mechanism Bacillus subtilis L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
3.2.1.99 1,5-alpha-L-arabinan + H2O F-11 and IFO 3022: best substrate, endo mechanism Bacillus subtilis F-11 L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
-
 ?
3.2.1.99 cell wall polysaccharide + H2O F-11, sycamore Bacillus subtilis L-arabinan
-
 ?
3.2.1.99 L-arabinan + H2O beet arabinan Bacillus subtilis L-arabino-oligosaccharides
-
 ?
3.2.1.99 L-arabinan + H2O random hydrolysis of L-arabinan Bacillus subtilis L-arabino-oligosaccharides
-
 ?
3.2.1.99 L-arabinan + H2O random hydrolysis of L-arabinan Clostridium felsineum var. sikokianum L-arabino-oligosaccharides
-
 ?
3.2.1.99 L-arabinan + H2O beet arabinan Bacillus subtilis F-11 L-arabino-oligosaccharides
-
 ?
3.2.1.99 additional information IFO 3022: potato disc macerating activity, able to loosen potato tissue Bacillus subtilis ?
-
 ?
3.2.1.99 additional information inactive towards phenyl and p-nitrophenyl alpha-L-arabinofuranoside, arabinoxylan, arabinogalactan, and gum arabic Bacillus subtilis ?
-
 ?
3.2.1.99 additional information IFO 3022: potato disc macerating activity, able to loosen potato tissue Bacillus subtilis F-11 ?
-
 ?
3.2.1.99 additional information inactive towards phenyl and p-nitrophenyl alpha-L-arabinofuranoside, arabinoxylan, arabinogalactan, and gum arabic Bacillus subtilis F-11 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 6
-
 F-11 Bacillus subtilis
3.2.1.99 6
-
 IFO 3022 Bacillus subtilis