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Literature summary extracted from

  • Nakakuki, T.; Hayashi, T.; Monma, M.; Kawashima, K.; Kainuma, K.
    Immobilization of the exo-maltohexaohydrolase by the irradiation method (1983), Biotechnol. Bioeng., 25, 1095-1107.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.98 synthesis continuous production of maltohexaose on large scale using the immobilized exomaltohexaohydrolase Klebsiella pneumoniae

General Stability

EC Number General Stability Organism
3.2.1.98 immobilized enzyme is very stable Klebsiella pneumoniae
3.2.1.98 immobilized enzyme stable to repeated use of 20 cycles Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.98 AgNO3 native and immobilized enzyme, immobilized enzyme less than native enzyme Klebsiella pneumoniae
3.2.1.98 Al2(SO4)3 immobilized enzyme, slight inactivation Klebsiella pneumoniae
3.2.1.98 Ba2+ immobilized enzyme Klebsiella pneumoniae
3.2.1.98 Cu2+ immobilized enzyme less than native enzyme Klebsiella pneumoniae
3.2.1.98 FeCl3 immobilized enzyme Klebsiella pneumoniae
3.2.1.98 FeSO4 native and immobilized enzyme Klebsiella pneumoniae
3.2.1.98 Hg2+ immobilized enzyme, 61% inactivation; native enzyme, completely Klebsiella pneumoniae
3.2.1.98 HgCl2 immobilized enzyme, 61% inactivation; native enzyme, completely Klebsiella pneumoniae
3.2.1.98 iodoacetamide native enzyme: 11% inhibition, immobilized enzyme: 4% inhibition Klebsiella pneumoniae
3.2.1.98 Mg2+ immobilized enzyme, slight inactivation Klebsiella pneumoniae
3.2.1.98 Mn2+ native enzyme, slight inactivation Klebsiella pneumoniae
3.2.1.98 additional information native enzyme compared with immobilized enzyme Klebsiella pneumoniae
3.2.1.98 p-chloromercuribenzoate native enzyme: 46% inhibition, immobilized enzyme: 15% inhibition Klebsiella pneumoniae
3.2.1.98 SrCl2 immobilized enzyme Klebsiella pneumoniae
3.2.1.98 Zn2+ native enzyme Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.98 additional information
-
 additional information native enzyme compared with immobilized enzyme, Km-value of native and immobilized enzyme for short-chain amylose is the same, for soluble starch Km-value of immobilized enzyme is larger than that of native enzyme Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.98 extracellular ultraviolet induced mutant Klebsiella pneumoniae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.98 Ca2+ native enzyme, activation Klebsiella pneumoniae
3.2.1.98 Ca2+ immobilized enzyme, no activation Klebsiella pneumoniae
3.2.1.98 CoCl2 native and immobilized enzyme, very slight activation Klebsiella pneumoniae
3.2.1.98 Mn2+ immobilized enzyme, activation Klebsiella pneumoniae
3.2.1.98 additional information native enzyme compared with immobilized enzyme Klebsiella pneumoniae
3.2.1.98 NiCl2 immobilized enzyme, slight activation Klebsiella pneumoniae
3.2.1.98 Sr2+ native enzyme, very slight activation Klebsiella pneumoniae
3.2.1.98 Zn2+ immobilized enzyme, very slight activation Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.98 Klebsiella pneumoniae
-
 Aerobacter aerogenes
-
3.2.1.98 Klebsiella pneumoniae
-
 native and immobilized, by irradiation method, enzyme
-
3.2.1.98 Klebsiella pneumoniae
-
 ultraviolet-induced mutant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.98 partial Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.98 15.6
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.98 amylose + H2O short-chain amylose, DP, average degree of polymerization, = 23 Klebsiella pneumoniae maltohexaose main product, about 38% maltohexaose ?
3.2.1.98 maltohexaose + H2O
-
 Klebsiella pneumoniae maltose + maltotetraose
-
 ?
3.2.1.98 maltohexaose + H2O
-
 Klebsiella pneumoniae D-glucose + maltopentaose
-
 ?
3.2.1.98 maltohexaose + H2O
-
 Klebsiella pneumoniae maltotriose + ?
-
 ?
3.2.1.98 additional information action pattern on substrates of native and immobilized enzyme is almost the same Klebsiella pneumoniae ?
-
 ?
3.2.1.98 starch + H2O
-
 Klebsiella pneumoniae maltohexaose + ? main product ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.98 additional information
-
 native enzyme compared with immobilized enzyme Klebsiella pneumoniae
3.2.1.98 52
-
 immobilized enzyme Klebsiella pneumoniae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.98 additional information
-
 native enzyme compared with immobilized enzyme Klebsiella pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.98 additional information
-
 thermostability is improved by immobilization Klebsiella pneumoniae
3.2.1.98 50
-
 immobilized enzyme, completely stable up to Klebsiella pneumoniae
3.2.1.98 75
-
 immobilized enzyme, 15 min at 75°C: 40% of activity retained Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.98 additional information
-
 native enzyme compared with immobilized enzyme, immobilized enzyme: optimum pH shifted slightly to acidic side Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.98 additional information
-
 native enzyme compared with immobilized enzyme Klebsiella pneumoniae
3.2.1.98 5 8 immobilized enzyme, more than 80% of maximum activity between 5.0 and 8.0 Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.98 additional information
-
 pH stability is improved by immobilization Klebsiella pneumoniae
3.2.1.98 3
-
 immobilized enzyme, 93% of activity retains Klebsiella pneumoniae
3.2.1.98 4 11 immobilized enzyme, stable between Klebsiella pneumoniae
3.2.1.98 11.5
-
 immobilized enzyme, 85% of activity retained Klebsiella pneumoniae