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Literature summary extracted from

  • Ogawa, K.; Uejima, O.; Nakakuki, T.; Usui, T.; Kainuma, K.
    Enzymatic synthesis of p-nitrophenyl alpha-maltoheptaoside by transglycosylation of maltohexaose-forming amylase (1990), Agric. Biol. Chem., 54, 581-586.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.98 ethanol transglycosylation, activation Klebsiella pneumoniae
3.2.1.98 methanol aqueous methanol system, up to 40% methanol, increases transglycosylation Klebsiella pneumoniae

Application

EC Number Application Comment Organism
3.2.1.98 synthesis enzymatic reaction, transglycosylation, provides practical technique for industrial production of p-nitrophenyl alpha-maltoheptaoside, useful substrate for assay of human alpha-amylase in serum and urine Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.98 1-propanol transglycosylation, inactivation Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.98 extracellular ultraviolet induced mutant Klebsiella pneumoniae
-
-

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.98 Ethanol stable and activation Klebsiella pneumoniae
3.2.1.98 Methanol stable up to 40%, at 50% methanol: inactivation of transglycosylation and hydrolysis Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.98 Klebsiella pneumoniae
-
 Aerobacter aerogenes
-
3.2.1.98 Klebsiella pneumoniae
-
 ultraviolet-induced mutant
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.98 (Glcalpha(1-4))m + H2O = (Glcalpha(1-4))m-6 + maltohexaose mechanism of transglycosylation Klebsiella pneumoniae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.98 amylose + H2O short-chain amylose, DP, average degree of polymerization, = 23 Klebsiella pneumoniae maltohexaose main product, 40% maltohexaose ?
3.2.1.98 maltoheptaose + p-nitrophenyl alpha-glucoside transglycosylation, mechanism Klebsiella pneumoniae p-nitrophenyl alpha-maltoheptaoside + D-glucose p-nitrophenyl alpha-maltoheptaoside useful substrate for assay of human alpha-amylase in serum and urine ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.98 G6-forming amylase
-
 Klebsiella pneumoniae
3.2.1.98 maltohexaose-forming amylase
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.98 35
-
 transglycosylation Klebsiella pneumoniae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.98 45
-
 transglycosylation, below, considerable inactivation at Klebsiella pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.98 45
-
 transglycosylation, considerabe inactivation Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.98 8
-
 transglycosylation Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.98 5 9 transglycosylation Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.98 4
-
 transglycosylation, complete inactivation Klebsiella pneumoniae