EC Number | General Stability | Organism |
---|---|---|
3.2.1.96 | 20-30% loss of initial activity is lost when the protein concentration is decreased to less than 0.02-0.03 mg/ml | Streptomyces plicatus |
3.2.1.96 | stable to lyophilization | Streptomyces plicatus |
3.2.1.96 | stable to repeated freezing and thawing | Streptomyces plicatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.96 | SDS | 0.2%, complete inactivation | Streptomyces plicatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.96 | 0.7 | - |
Man(GlcNAc)2Asn-dansyl | - |
Streptomyces plicatus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.96 | 46000 | - |
gel filtration | Streptomyces plicatus |
3.2.1.96 | 49500 | - |
equilibrium sedimentation | Streptomyces plicatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.96 | Streptomyces plicatus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.96 | - |
Streptomyces plicatus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.96 | additional information | - |
- |
Streptomyces plicatus |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.96 | 4°C, stable for up to 6 months | Streptomyces plicatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.96 | beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-Asn | - |
Streptomyces plicatus | beta-D-Man-(1->4)-beta-D-GlcNAc + beta-D-GlcNAc-Asn | - |
? | |
3.2.1.96 | Man(GlcNAc)2Asn-dansyl + H2O | - |
Streptomyces plicatus | ? | - |
? | |
3.2.1.96 | N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Streptomyces plicatus | 2 N,N'-diacetylchitobiose | - |
? | |
3.2.1.96 | N,N',N''-triacetylchitotriose + H2O | - |
Streptomyces plicatus | N-acetyl-D-glucosamine + N,N'-diacetylchiotobiose | - |
? | |
3.2.1.96 | N4-(beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc)-N2-dansyl-L-Asn + H2O | - |
Streptomyces plicatus | beta-D-Man-(1->4)-beta-D-GlcNAc + N4-(beta-D-GlcNAc)-N2-dansyl-L-Asn | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.96 | monomer | 1 * 52000-54000, SDS-PAGE | Streptomyces plicatus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.96 | endo-beta-N-acetylglucosaminidase L | - |
Streptomyces plicatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.96 | 4 | 4.5 | hydrolysis of Man(GlcNAc)2Asn-dansyl | Streptomyces plicatus |