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Literature summary extracted from

  • Rogers, H.J.; Taylor, C.; Rayter, S.; Ward, J.B.
    Purification and properties of autolytic endo-beta-N-acetylglucosaminidase and the N-acetylmuramyl-L-alanine amidase from Bacillus subtilis strain 168 (1984), J. Gen. Microbiol., 130, 2395-2402.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.96 trypsin, 0.01 mg/ml, pH 7.2, 50 mM Tris/HCl, 70% inactivation after 15 min Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.96 lipoteichoic acid
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.96 Li+ maximal activation near 0.2 M LiCl with Micrococcus luteus cell walls as substrate, sharp optimal activation at 0.1 M with Bacillus subtilis cell walls as substrate Bacillus subtilis
3.2.1.96 Mg2+ slight activation Bacillus subtilis
3.5.1.28 Mg2+ enzyme activated by low concentrations of Mg2+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.96 90000
-
 2 * 90000, SDS-PAGE Bacillus subtilis
3.2.1.96 190000
-
 gel filtration Bacillus subtilis
3.5.1.28 30000 40000 gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Bacillus subtilis
-
-
-
3.2.1.96 Bacillus subtilis 168
-
-
-
3.5.1.28 Bacillus subtilis
-
-
-
3.5.1.28 Bacillus subtilis 168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.96
-
 Bacillus subtilis
3.5.1.28
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.96 additional information
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 additional information no exo-activity Bacillus subtilis ?
-
 ?
3.2.1.96 additional information no exo-activity Bacillus subtilis 168 ?
-
 ?
3.2.1.96 peptidoglycan + H2O Micrococcus luteus cell wall and Bacillus subtilis cell walls Bacillus subtilis ?
-
 ?
3.2.1.96 peptidoglycan + H2O Micrococcus luteus cell wall and Bacillus subtilis cell walls Bacillus subtilis 168 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.96 dimer 2 * 90000, SDS-PAGE Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.96 additional information
-
 additional information
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 4.5
-
 Bacillus subtilis cell walls Bacillus subtilis
3.2.1.96 4.5 5.5 Micrococcus luteus cell walls as substrate, in 50 mM acetic acid/acetate buffer or succinate/NaOH buffer Bacillus subtilis
3.2.1.96 5
-
-
 Bacillus subtilis
3.5.1.28 7.5
-
 phosphate buffer Bacillus subtilis
3.5.1.28 8.6
-
 tetraborate/boric acid buffer Bacillus subtilis