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Literature summary extracted from

  • Hitomi, J.; Murakami, Y.; Saitoh, F.; Shigemitsu, N.; Yamaguchi, H.
    Purification and characterization of endo-beta-N-acetylglucosaminidase of Aspergillus oryzae (1985), J. Biochem., 98, 527-533.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.96 dithiothreitol 1 mM, 18% stimulation Aspergillus oryzae
3.2.1.96 L-Cys 1 mM, 18% stimulation Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.96 0.0868
-
 dansyl derivative of Taka-amylase A glycopeptide
-
 Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.96 31000
-
 gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Aspergillus oryzae
-
-
-
3.2.1.96 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.96
-
 Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.96 additional information
-
-
 Aspergillus oryzae

Storage Stability

EC Number Storage Stability Organism
3.2.1.96 stable for at least 4 months in the refrigerator at pH 4.0-6.0 Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 dansyl derivative of Taka-amylase A glycopeptide + H2O
-
 Aspergillus oryzae ?
-
 ?
3.2.1.96 additional information
-
 Streptomyces griseus ?
-
 ?
3.2.1.96 additional information high-mannose-type glycopeptides are rapidly hydrolyzed, hybrid-type glycopeptides are good substrates, complex-type glycopeptides as well as smaller glycopeptides prepared by partial glycosidase digestion of complex-type glycopeptides are resistant to the enzyme Aspergillus oryzae ?
-
 ?
3.2.1.96 Taka-amylase A glycopeptide + H2O dansyl derivative of taka-amylase A glycopeptide Aspergillus oryzae additional information product contains N-acetylglucosaminitol and mannose in the molar ratio 1.0:5.2, but no N-acetylglucosamine or mannitol ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.96 50
-
 pH 5.0, 0.1 M acetate buffer, 2 h, less than 10% loss of activity Aspergillus oryzae
3.2.1.96 60
-
 10 min, more than 90% loss of activity Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 4 4.8 hydrolysis of dansyl derivative of Taka-amylase A glycopeptide Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.96 4 6 in the refrigerator, stable for at least 4 months Aspergillus oryzae