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Literature summary extracted from

  • Breccia, J.D.; Sineriz, F.; Baigori, M.D.; Castro, G.R.; Hatti-Kaul, R.
    Purification and characterization of a thermostable xylanase from Bacillus amyloliquefaciens (1998), Enzyme Microb. Technol., 22, 42-49.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 beta-mercaptoethanol stimulates Bacillus amyloliquefaciens
3.2.1.8 dithiothreitol stimulates Bacillus amyloliquefaciens
3.2.1.8 EDTA 1-5 mM, stimulates Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+
-
 Bacillus amyloliquefaciens
3.2.1.8 Fe3+
-
 Bacillus amyloliquefaciens
3.2.1.8 Hg2+
-
 Bacillus amyloliquefaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Bacillus amyloliquefaciens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Mn2+ stimulates Bacillus amyloliquefaciens
3.2.1.8 Mn2+ maximal activity at 2 mM Bacillus amyloliquefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 18500
-
 equilibrium sedimentation Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Bacillus amyloliquefaciens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.8 glycoprotein the enzyme is either a glycoprotein or possesses a tightly bound carbohydrate residue Bacillus amyloliquefaciens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Bacillus amyloliquefaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 213.1
-
-
 Bacillus amyloliquefaciens

Subunits

EC Number Subunits Comment Organism
3.2.1.8 additional information two protein bands are detected by SDS-PAGE: 18400 Da and 19600 Da Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 80
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 40 90 40°C: about 50% of maximal activity, 90°C: about 85% of maximal activity Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 stable for several days Bacillus amyloliquefaciens
3.2.1.8 55
-
 pH 6.8, 15 min, stable up to Bacillus amyloliquefaciens
3.2.1.8 75
-
 pH 6.8, 15 min, nearly all activity is destroyed Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.8 7
-
 Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 3 10 about 40% of maximal activity at pH 3.0 and at pH 10.0 Bacillus amyloliquefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 9
-
 50°C, 5 h, stable Bacillus amyloliquefaciens
3.2.1.8 10
-
 50°C, 5 h, less than 20% loss of activity Bacillus amyloliquefaciens