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Literature summary extracted from

  • Bataillon, M.; Cardinali, A.P.N.; Castillon, N.; Duchiron, F.
    Purification and characterization of a moderately thermostable xylanase from Bacillus sp. strain SPS-0 (2000), Enzyme Microb. Technol., 26, 187-192.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 beta-mercaptoethanol 10 mM, stimulates Bacillus sp. (in: Bacteria)
3.2.1.8 dithiothreitol stimulates Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 5,5'-dithiobis-(2-nitrobenzoic acid)
-
 Bacillus sp. (in: Bacteria)
3.2.1.8 Cu2+ 1 mM, 30% loss of activity Bacillus sp. (in: Bacteria)
3.2.1.8 DTNB
-
 Bacillus sp. (in: Bacteria)
3.2.1.8 Hg2+ 1 mM, complete inhibition Bacillus sp. (in: Bacteria)
3.2.1.8 N-bromosuccinimide
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Mn2+ stimulates Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 99000
-
 x * 99000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Bacillus sp. (in: Bacteria)
-
-
-
3.2.1.8 Bacillus sp. (in: Bacteria) SPS-0
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 1,4-beta-D-xylan + H2O birchwood xylan Bacillus sp. (in: Bacteria) additional information xylose + xylobiose + xylotriose + xylotetraose ?
3.2.1.8 1,4-beta-D-xylan + H2O birchwood xylan Bacillus sp. (in: Bacteria) SPS-0 additional information xylose + xylobiose + xylotriose + xylotetraose ?
3.2.1.8 arabinoxylan + H2O from wheat bran Bacillus sp. (in: Bacteria) additional information
-
 ?
3.2.1.8 arabinoxylan + H2O from wheat bran Bacillus sp. (in: Bacteria) SPS-0 additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 99000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 75
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 60 80 60°C: about 50% of maximal activity, 80°C: about 80% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 60
-
 stable Bacillus sp. (in: Bacteria)
3.2.1.8 70
-
 pH 6.0, 4 h, 20% loss of activity in presence of xylan, 80% loss of activity without xylan Bacillus sp. (in: Bacteria)
3.2.1.8 70
-
 4 h, in presence of xylan, 20% loss of activity Bacillus sp. (in: Bacteria)
3.2.1.8 80
-
 15 min, complete loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6 7
-
 Bacillus sp. (in: Bacteria)
3.2.1.8 6
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 9 pH 5.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity Bacillus sp. (in: Bacteria)