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Literature summary extracted from

  • Lin, J.; Ndlovu, L.M.; Singh, S.; Pillay, B.
    Purification and biochemical characteristics of beta-D-xylanase from a thermophilic fungus, Thermomyces lanuginosus - SSBP (1999), Biotechnol. Appl. Biochem., 30, 73-79.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 paper production potential use in biopulping processes Thermomyces lanuginosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 5,5'-dithiobis-(2-nitrobenzoic acid)
-
 Thermomyces lanuginosus
3.2.1.8 beta-mercaptoethanol
-
 Thermomyces lanuginosus
3.2.1.8 diethyl dicarbonate
-
 Thermomyces lanuginosus
3.2.1.8 dithiothreitol
-
 Thermomyces lanuginosus
3.2.1.8 DTNB
-
 Thermomyces lanuginosus
3.2.1.8 Hg2+ 6 mM, complete inhibition Thermomyces lanuginosus
3.2.1.8 iodoacetamide
-
 Thermomyces lanuginosus
3.2.1.8 N-bromosuccinimide
-
 Thermomyces lanuginosus
3.2.1.8 Pb2+ 8 mM, 26% loss ofv activity Thermomyces lanuginosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Thermomyces lanuginosus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 12000
-
 gel filtration Thermomyces lanuginosus
3.2.1.8 23600
-
 x * 23600, SDS-PAGE Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Thermomyces lanuginosus
-
-
-
3.2.1.8 Thermomyces lanuginosus SSBP
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
 Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 additional information
-
-
 Thermomyces lanuginosus
3.2.1.8 3209
-
-
 Thermomyces lanuginosus

Storage Stability

EC Number Storage Stability Organism
3.2.1.8 4°C, retains full activity after 9 months Thermomyces lanuginosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 1,4-beta-D-xylan + H2O
-
 Thermomyces lanuginosus additional information
-
 ?
3.2.1.8 1,4-beta-D-xylan + H2O
-
 Thermomyces lanuginosus SSBP additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 23600, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 70 75
-
 Thermomyces lanuginosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 50 85 about 50% of maximal activity at 50°C and at 85°C Thermomyces lanuginosus
3.2.1.8 70 75
-
 Thermomyces lanuginosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 60
-
 stable for more than 48 h Thermomyces lanuginosus
3.2.1.8 75
-
 30 min, 80% loss of activity Thermomyces lanuginosus
3.2.1.8 90
-
 30 min, complete inactivation Thermomyces lanuginosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
-
 Thermomyces lanuginosus
3.2.1.8 6.5 7
-
 Thermomyces lanuginosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 5
-
 stable up to Thermomyces lanuginosus
3.2.1.8 5 12 stable Thermomyces lanuginosus