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Literature summary extracted from

  • Tan, L.U.L.; Mayers, P.; Saddler, J.N.
    Purification and characterization of a thermostable xylanase from a thermophilic fungus Thermoascus aurantiacus (1987), Can. J. Microbiol., 33, 689-692.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Hg2+ 2 mM, complete loss of activity; 2 mM HgCl2, complete inactivation Thermoascus aurantiacus
3.2.1.8 additional information the xylanase was not affected by copper sulfate, zinc sulfate, calcium chloride, cobalt chloride, barium chloride, magnesium sulfate, and EDTA at concentrations of 2 mM Thermoascus aurantiacus
3.2.1.8 Pb(CH3COO)2 2 mM, activity is reduced by 25% Thermoascus aurantiacus
3.2.1.8 Pb2+ 2 mM, 25% loss of activity Thermoascus aurantiacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
additional information
-
Thermoascus aurantiacus
3.2.1.8 additional information
-
xylan 0.17% w/v, 50°C Thermoascus aurantiacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 32000
-
-
Thermoascus aurantiacus
3.2.1.8 32000
-
1 * 32000, SDS-PAGE Thermoascus aurantiacus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Thermoascus aurantiacus
-
-
-
3.2.1.8 Thermoascus aurantiacus C436
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8
-
Thermoascus aurantiacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 42.4
-
-
Thermoascus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 1,4-beta-D-xylan + H2O
-
Thermoascus aurantiacus additional information
-
?
3.2.1.8 1,4-beta-D-xylan + H2O
-
Thermoascus aurantiacus C436 additional information
-
?
3.2.1.8 xylan + H2O
-
Thermoascus aurantiacus ?
-
?
3.2.1.8 xylan + H2O
-
Thermoascus aurantiacus C436 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 32000, SDS-PAGE Thermoascus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 80
-
-
Thermoascus aurantiacus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 70 85 70°C: about 70% of maximal activity, 85°C: about 10% of maximal activity Thermoascus aurantiacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 60
-
97 h, retains full activity Thermoascus aurantiacus
3.2.1.8 60
-
retains full activity for 97 h Thermoascus aurantiacus
3.2.1.8 70
-
24 h, retains full activity Thermoascus aurantiacus
3.2.1.8 70
-
retains full activity for 24 h Thermoascus aurantiacus
3.2.1.8 80
-
half-life: 54 min Thermoascus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5.1
-
-
Thermoascus aurantiacus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 3.5 7.5 12% of maximal activity at pH 3.5 and at pH 7.5 Thermoascus aurantiacus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Thermoascus aurantiacus
-
-
7.1