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Literature summary extracted from

  • Olsen, O.; Borrisss, R.; Simon, O.; Thomsen, K.K.
    Hybrid Bacillus (1-3,1-4)-beta-glucanases: engineering thermostable enzymes by construction of hybrid genes (1991), Mol. Gen. Genet., 225, 177-185.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli Bacillus amyloliquefaciens
3.2.1.73 hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli Paenibacillus macerans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.73 additional information hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli Bacillus amyloliquefaciens
3.2.1.73 additional information hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, expression in Escherichia coli Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Bacillus amyloliquefaciens
-
 parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase
-
3.2.1.73 Bacillus subtilis
-
-
-
3.2.1.73 Paenibacillus macerans
-
 parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase Bacillus amyloliquefaciens
3.2.1.73 parental enzymes and hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase Paenibacillus macerans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 3722
-
-
 Bacillus amyloliquefaciens
3.2.1.73 3722
-
-
 Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 beta-D-glucan + H2O beta-D-glucan from barley Bacillus subtilis additional information
-
 ?
3.2.1.73 beta-D-glucan + H2O beta-D-glucan from barley Bacillus amyloliquefaciens additional information
-
 ?
3.2.1.73 beta-D-glucan + H2O beta-D-glucan from barley Paenibacillus macerans additional information
-
 ?
3.2.1.73 lichenin + H2O
-
 Bacillus subtilis additional information
-
 ?
3.2.1.73 lichenin + H2O
-
 Bacillus amyloliquefaciens additional information
-
 ?
3.2.1.73 lichenin + H2O
-
 Paenibacillus macerans additional information
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 55
-
-
 Bacillus amyloliquefaciens
3.2.1.73 55
-
-
 Bacillus subtilis
3.2.1.73 65
-
-
 Paenibacillus macerans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.73 37 65 more than 80% of maximal activity at 37°C and at 65°C Bacillus subtilis
3.2.1.73 37 65 more than 80% of maximal activity at 37°C and at 65°C Bacillus amyloliquefaciens
3.2.1.73 55 75 more than 80% of maximal activity at 55°C and at 75°C Paenibacillus macerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 70
-
 pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity Bacillus amyloliquefaciens
3.2.1.73 70
-
 pH 6.0, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase exhibit 90% of the initial activity, the parental enzymes retain 5% or less of the initial activity Paenibacillus macerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 6.5
-
-
 Bacillus subtilis
3.2.1.73 6.5
-
-
 Bacillus amyloliquefaciens
3.2.1.73 7.6
-
-
 Bacillus subtilis
3.2.1.73 7.6
-
-
 Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 5.5 7 more than 80% of maximal activity at pH 5.5 and pH 7.0 Bacillus amyloliquefaciens
3.2.1.73 5.9 6.5 more than 80% of maximal activity at pH 5.9 and pH 6.5 Bacillus subtilis
3.2.1.73 5.9 8.5 more than 80% of maximal activity at pH 5.9 and pH 8.5 Paenibacillus macerans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 4.1
-
 65°C, 1 h, 95% loss of activity Bacillus amyloliquefaciens
3.2.1.73 4.1
-
 65°C, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, 85%-95% of the initial activity is retained Bacillus amyloliquefaciens
3.2.1.73 4.1
-
 65°C, 1 h, hybrid enzymes containing 16, 36, 78, or 152 amino acid N-terminal sequence derived from Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan glucanohydrolase followed by a C-terminal segment derived from Bacillus macerans 1,3-1,4-beta-D-glucan glucanohydrolase, 85%-95% of the initial activity is retained Paenibacillus macerans
3.2.1.73 4.1
-
 65°C, 1 h, complete inactivation Paenibacillus macerans