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Literature summary extracted from

  • Akiyama, T.; Kaku, H.; Shibuya, N.
    Purification, characterization and NH2-terminal sequencing of an endo-(1->3,1->4)-beta-glucanase from rice (Oryza sativa L.) bran (1998), Plant Sci., 134, 3-10.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 31000
-
 1 * 31000, SDS-PAGE Oryza sativa
3.2.1.73 32000
-
 gel filtration Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Oryza sativa
-
 L. cv. Kirara 397
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73
-
 Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 387
-
-
 Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 beta-D-glucan + H2O beta-D-glucan from barley Oryza sativa additional information oligosaccharides are formed as the major products, no glucose ?
3.2.1.73 beta-D-glucan + H2O beta-D-glucan from oat Oryza sativa additional information oligosaccharides are formed as the major products, no glucose ?
3.2.1.73 lichenin + H2O lichenin from Cetralia islandica and from Usnea barbata Oryza sativa additional information
-
 ?
3.2.1.73 additional information no hydrolysis of laminarin Oryza sativa ?
-
 ?
3.2.1.73 additional information no hydrolysis of carboxymethylcellulose Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 monomer 1 * 31000, SDS-PAGE Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 40
-
 10 min, stable Oryza sativa
3.2.1.73 50
-
 10 min, about 25% loss of activity Oryza sativa
3.2.1.73 60
-
 10 min, complete inactivation Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 4.5
-
-
 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 3 6 pH 3.0: about 50% of maximal activity, pH 6.0: about 40% of maximal activity Oryza sativa