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Literature summary extracted from

  • Chen, H.; Li, X.L.; Ljungdahl, L.G.
    Sequencing of a 1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin (1997), J. Bacteriol., 179, 6028-6034.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 expression in Escherichia coli Orpinomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
 additional information
-
 Orpinomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.73 extracellular recombinant enzyme expressed in Escherichia coli Orpinomyces sp.
-
-
3.2.1.73 periplasmic space
-
 Orpinomyces sp. 42597
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 27000
-
 x * 27000, recombinant enzyme, SDS-PAGE Orpinomyces sp.
3.2.1.73 27929
-
 x * 27929, calculation from nucleotide sequence Orpinomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Orpinomyces sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.73 proteolytic modification a N-terminal signal peptide of 29 amino residues is cleaved from the enzyme secreted from Orpinomyces whereas 21 amino acid residues of the signal peptide are removed when the enzyme is produced by E. coli Orpinomyces sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 recombinant enzyme Orpinomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.73 culture medium recombinant enzyme expressed in E. coli Orpinomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 3659
-
-
 Orpinomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 beta-D-glucan + H2O beta-D-glucan from barley Orpinomyces sp. additional information main products are triose and tetraose ?
3.2.1.73 lichenin + H2O
-
 Orpinomyces sp. additional information main products are triose and tetraose ?
3.2.1.73 additional information no hydrolysis of xylan Orpinomyces sp. ?
-
 ?
3.2.1.73 additional information no hydrolysis of laminarin Orpinomyces sp. ?
-
 ?
3.2.1.73 additional information no hydrolysis of carboxymethylcellulose Orpinomyces sp. ?
-
 ?
3.2.1.73 additional information no hydrolysis of pustulan Orpinomyces sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x * 27000, recombinant enzyme, SDS-PAGE Orpinomyces sp.
3.2.1.73 ? x * 27929, calculation from nucleotide sequence Orpinomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 45
-
-
 Orpinomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 40
-
 recombinant enzyme, pH 6.0, 24 h, stable Orpinomyces sp.
3.2.1.73 45
-
 recombinant enzyme, pH 6.0, 24 h, 28% loss of activity Orpinomyces sp.
3.2.1.73 50
-
 recombinant enzyme, pH 6.0, 24 h, 41% loss of activity Orpinomyces sp.
3.2.1.73 55
-
 recombinant enzyme, pH 6.0, 24 h, 70% loss of activity Orpinomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 5.8 6.2
-
 Orpinomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 5.4 7 about 80% of maximal activity at pH 5.4 and at pH 7.0 Orpinomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 3.4 9.8 4°C, stable for at least 24 h Orpinomyces sp.