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Literature summary extracted from

  • Suzuki, H.; Kaneko, T.
    Degradation of barley glucan and lichenan by a Bacillus pumilus enzyme (1976), Agric. Biol. Chem., 40, 577-586.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.73 Hg2+
-
Bacillus pumilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 12000
-
discrepancy between molecular weight determined by gel filtration and low speed equilibrium sedimentation is probably due to a moderate interaction between the enzyme and the Sephadex polmer, gel filtration Bacillus pumilus
3.2.1.73 27000
-
discrepancy between molecular weight determined by gel filtration and low speed equilibrium sedimentation is probably due to a moderate interaction between the enzyme and the Sephadex polmer, low speed sedimentation without reaching equilibrium Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Bacillus pumilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73
-
Bacillus pumilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 additional information
-
-
Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 beta-D-glucan + H2O beta-D-glucan from barley Bacillus pumilus additional information
-
?
3.2.1.73 lichenan + H2O
-
Bacillus pumilus ?
-
?
3.2.1.73 additional information no hydrolysis of laminaran Bacillus pumilus ?
-
?
3.2.1.73 additional information no hydrolysis of carboxymethylcellulose Bacillus pumilus ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 50 60
-
Bacillus pumilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 60
-
10 min, about 35% loss of activity Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 7
-
-
Bacillus pumilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 5 7 10 min, stable Bacillus pumilus