EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.73 | expression in Escherichia coli | Fibrobacter succinogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.73 | additional information | - |
additional information | - |
Fibrobacter succinogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 35200 | - |
1 * 35200, SDS-PAGE | Fibrobacter succinogenes |
3.2.1.73 | 37200 | - |
gel filtration | Fibrobacter succinogenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.73 | Fibrobacter succinogenes | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.73 | - |
Fibrobacter succinogenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 1372 | - |
- |
Fibrobacter succinogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.73 | beta-D-glucan + H2O | beta-D-glucan from oat | Fibrobacter succinogenes | additional information | - |
? | |
3.2.1.73 | lichenin + H2O | - |
Fibrobacter succinogenes | additional information | - |
? | |
3.2.1.73 | additional information | no hydrolysis of carboxymethylpachyman | Fibrobacter succinogenes | ? | - |
? | |
3.2.1.73 | additional information | no hydrolysis of starch | Fibrobacter succinogenes | ? | - |
? | |
3.2.1.73 | additional information | no hydrolysis of xylan | Fibrobacter succinogenes | ? | - |
? | |
3.2.1.73 | additional information | no hydrolysis of laminarin | Fibrobacter succinogenes | ? | - |
? | |
3.2.1.73 | additional information | no hydrolysis of carboxymethylcellulose | Fibrobacter succinogenes | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.73 | monomer | 1 * 35200, SDS-PAGE | Fibrobacter succinogenes |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 50 | - |
lichenin or oat beta-D-glucan as substrate | Fibrobacter succinogenes |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 30 | 60 | 30°C, about 30% of maximal activity with oat beta-D-glucan, about 45% of maximal activity with lichenin, 60°C: about 20% of maximal activity with lichenin and oat beta-D-glucan as substrate | Fibrobacter succinogenes |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 60 | - |
10 min, 50% loss of activity, crude enzyme extract | Fibrobacter succinogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 5.8 | 6.3 | - |
Fibrobacter succinogenes |