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Literature summary extracted from

  • Bates, E.J.; Heaton, G.M.; Taylor, C.; Kernohan, J.C.; Cohen, P.
    Debranching enzyme from rabbit skeletal muscle: evidence for the location of two active centres on a single polypeptide chain (1975), FEBS Lett., 58, 181-185.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.33 glucosidase: rather resistant to proteolytic attack: very little inactivation after prolonged incubation with 1% trypsin or 1% chymotrypsin Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.33 164000
-
 glycogen debranching enzyme, sedimentation equilibrium Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 glycogen debranching enzyme Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 muscle skeletal Oryctolagus cuniculus
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.33 0°C, 50 mM glycerophosphate, 2 mM EDTA, 1 mM dithiothreitol, pH 7.0, 2 months, without loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 glycogen + H2O reverse reaction: incorporation of glucose into glycogen Oryctolagus cuniculus glycogen + D-glucose
-
 r
3.2.1.33 glycogen + H2O slight reversibility Oryctolagus cuniculus glycogen + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose
-
 r

Subunits

EC Number Subunits Comment Organism
3.2.1.33 monomer
-
 Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.33 25
-
 completely stable for 20 h at 25°C in 50 mM glycerophosphate, 2 mM EDTA, 1 mM dithiothreitol, pH 7.0 Oryctolagus cuniculus