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Literature summary extracted from

  • Takrama, J.; Madsen, N.B.
    Binding of glycogen, oligosaccharides, and glucose to glycogen debranching enzyme (1988), Biochemistry, 27, 3308-3314.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.33 5,5'-dithiobis(2-nitrobenzoic acid) DTNB Oryctolagus cuniculus
3.2.1.33 5-amino-D-glucose nojirimycin, potent inhibitor, noncompetitive Oryctolagus cuniculus
3.2.1.33 maltotriose competitive Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.33 additional information
-
 additional information kinetic data Oryctolagus cuniculus
3.2.1.33 additional information
-
 additional information kinetic mechanism Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O Oryctolagus cuniculus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 glycogen debranching enzyme Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.33 6-O-alpha-D-glucosyl cyclomaltoheptaose + H2O = D-glucose + cyclomaltoheptaose mechanism Oryctolagus cuniculus
a
3.2.1.33 6-O-alpha-D-glucosyl cyclomaltoheptaose + H2O = D-glucose + cyclomaltoheptaose thermodynamic data Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 muscle skeletal Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 11
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 63-alpha-glucosyl maltotetraose + H2O branched pentasaccharide "fast B5" Oryctolagus cuniculus maltotetraose + D-glucose
-
 r
3.2.1.33 alpha-(1-6)-glucosyl cyclohexaamylose + H2O 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin Oryctolagus cuniculus cyclohexaamylose + D-glucose
-
 r
3.2.1.33 glycogen + H2O reverse reaction: incorporation of glucose into glycogen Oryctolagus cuniculus glycogen + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.33 monomer
-
 Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus