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Literature summary extracted from

  • Becker, J.U.; Long, T.J.; Fischer, E.H.
    Purification and properties of debranching enzyme from dogfish muscle (1977), Biochemistry, 16, 291-297.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.33 additional information no activating by usual sugar phosphates or nucleotide effectors of glycolysis such as AMP, ADP, ATP, uridine diphosphoglucose, glucose-1-phosphate, glucose-6-phosphate and phosphate Squalus acanthias

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.33 5,5'-dithiobis(2-nitrobenzoic acid) 50% inhibition, reversed by glycogen Squalus acanthias
3.2.1.33 additional information no inhibition by usual sugar phosphates or nucleotide effectors of glycolysis such as AMP, ADP, ATP, uridine diphosphoglucose, glucose-1-phosphate, glucose-6-phosphate and phosphate Squalus acanthias
3.2.1.33 Tris 15 mM: 75% inhibition Squalus acanthias

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.33 160000
-
glycogen debranching enzyme, PAGE and SDS-PAGE Squalus acanthias
3.2.1.33 162000
-
glycogen debranching enzyme, carboxymethylation prior to sedimentation equilibrium Squalus acanthias

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.33 additional information Squalus acanthias glycogen debranching enzyme might play an important role in the structure of glycogen particles, or the control of its metabolism ?
-
?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.33 Ethanol stable in ethanol precipitation with final concentration of 23% ethanol Squalus acanthias

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Squalus acanthias
-
pacific dogfish
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.33 no modification no covalently bound phosphate or carbohydrate, method would not have detected less than 5% carbohydrate Squalus acanthias

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 glycogen debranching enzyme Squalus acanthias

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.33 6-O-alpha-D-glucosyl cyclomaltoheptaose + H2O = D-glucose + cyclomaltoheptaose regulation of enzyme activity Squalus acanthias

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 muscle
-
Squalus acanthias
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 3
-
maleate buffer, pH 6.0, 19°C Squalus acanthias

Storage Stability

EC Number Storage Stability Organism
3.2.1.33 frozen, solution in Tris-glycerol buffer, 3 months, without loss of activity Squalus acanthias

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 alpha-(1-6)-glucosyl cyclohexaamylose + H2O
-
Squalus acanthias cyclohexaamylose + D-glucose
-
r
3.2.1.33 glycogen + H2O liver glycogen and shellfish glycogen: one-tenth of activity compared with glycogen phosphorylase limit dextrin as substrate Squalus acanthias glycogen + D-glucose
-
r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
Squalus acanthias limit dextrin + D-glucose
-
r
3.2.1.33 additional information glycogen debranching enzyme might play an important role in the structure of glycogen particles, or the control of its metabolism Squalus acanthias ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.33 monomer 1 * 160000, glycogen debranching enzyme, carboxymethylation prior to SDS-PAGE Squalus acanthias

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Squalus acanthias

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.33 19
-
-
Squalus acanthias

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.33 20
-
unstable above Squalus acanthias
3.2.1.33 30
-
rapid inactivation Squalus acanthias

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.33 5.5 6.3
-
Squalus acanthias

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.33 4.6 9 inactive below pH 4.6 and above pH 9 Squalus acanthias