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Literature summary extracted from

  • Watts, T.E.; Nelson, T.E.
    Improved purification procedure for rabbit muscle amylo-1,6-glucosidase/oligo-1,4 -> 1,4-glucantransferase (1972), Anal. Biochem., 49, 479-491.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.33 glucosidase-transferase stable in 0.05 M Tris and 2 M urea, up to 30°C Oryctolagus cuniculus
3.2.1.33 glucosidase-transferase stable in 2 M urea, 2 M urea for prolonged periods of time: no loss in activity Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 glycogen debranching enzyme Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 8.78
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 63-alpha-glucosyl maltotetraose + H2O branched pentasaccharide "fast B5" Oryctolagus cuniculus maltotetraose + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O
-
 Oryctolagus cuniculus limit dextrin + D-glucose
-
 r

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.33 30
-
 up to, in the presence of Tris and urea Oryctolagus cuniculus
3.2.1.33 45
-
 complete loss of activity Oryctolagus cuniculus