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Literature summary extracted from

  • Gillard, B.K.; White, R.C.; Zingaro, R.A.; Nelson, T.E.
    Amylo-1,6-glucosidase/4-alpha-glucanotransferase. Reaction of rabbit muscle debranching enzyme with an active site-directed irreversible inhibitor, 1-S-dimethylarsino-1-thio-beta-D-glucopyranoside (1980), J. Biol. Chem., 255, 8451-8457.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.33 1-S-dimethylarsino-1-thio-beta-D-glucanopyranoside DATG, active site-directed irreversible inhibitor, inhibition: mechanism. Inactivation occurs with significant conformational change Mammalia
3.2.1.33 1-S-dimethylarsino-1-thio-beta-D-glucanopyranoside DATG, active site-directed irreversible inhibitor, inhibition: mechanism. Inactivation occurs with significant conformational change; glycogen phosphorylase limit dextrin and Bis-Tris protect from inactivation Oryctolagus cuniculus
3.2.1.33 2,2-bis(hydroxymethyl)-2,2',2''-nitrilotriethanol Bis-Tris, reversible inhibitor Oryctolagus cuniculus
3.2.1.33 cyclohexaamylose alpha-Schardinger dextrin, debranching enzyme, competitive inhibitor Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.33 additional information
-
 additional information kinetic mechanism Oryctolagus cuniculus
3.2.1.33 43
-
 D-glucose
-
 Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O Mammalia phi-dextrin ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O Oryctolagus cuniculus phi-dextrin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Mammalia
-
-
-
3.2.1.33 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 glycogen debranching enzyme Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.33 6-O-alpha-D-glucosyl cyclomaltoheptaose + H2O = D-glucose + cyclomaltoheptaose mechanism Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 muscle
-
 Mammalia
-
3.2.1.33 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 6.5 8.9
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
3.2.1.33 4°C, 50 mM Tris, 5 mM EDTA, 1 mM dithiothreitol, pH 7.2 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 63-alpha-glucosyl maltotetraose + H2O branched pentasaccharide "fast B5" Mammalia maltotetraose + D-glucose
-
 r
3.2.1.33 63-alpha-glucosyl maltotetraose + H2O branched pentasaccharide "fast B5" Oryctolagus cuniculus maltotetraose + D-glucose
-
 r
3.2.1.33 alpha-(1-6)-glucosyl cyclohexaamylose + H2O 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin Mammalia cyclohexaamylose + D-glucose alpha-Schardinger dextrin r
3.2.1.33 alpha-(1-6)-glucosyl cyclohexaamylose + H2O 6-alpha-glucosyl alpha-Schardinger dextrin, cyclodextrin Oryctolagus cuniculus cyclohexaamylose + D-glucose alpha-Schardinger dextrin r
3.2.1.33 glycogen + H2O
-
 Mammalia glycogen + D-glucose
-
 r
3.2.1.33 glycogen + H2O reverse reaction: synthesis of (1, 6)-bound side chains, reincorporation of glucose into polysaccharide Oryctolagus cuniculus glycogen + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Mammalia limit dextrin + D-glucose
-
 r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Oryctolagus cuniculus limit dextrin + D-glucose debranched phi-dextrin r
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Mammalia ?
-
 ?
3.2.1.33 glycogen phosphorylase-limit dextrin + H2O phi-dextrin Oryctolagus cuniculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Mammalia
3.2.1.33 glycogen debranching system EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system Oryctolagus cuniculus