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Literature summary extracted from

  • Nakao, M.; Harada, M.; Kodama, Y.; Nakayama, T.; Shibano, Y.; Amachi, T.
    Purification and characterization of a thermostable beta-galactosidase with high transgalactosylation activity from Saccharopolyspora rectivirgula (1994), Appl. Microbiol. Biotechnol., 40, 657-663.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.23 nutrition enzyme potentially serves as an inexpensive beta-galactosidase in food industry Saccharopolyspora rectivirgula

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ag+
-
 Saccharopolyspora rectivirgula
3.2.1.23 Cu2+
-
 Saccharopolyspora rectivirgula
3.2.1.23 D-galactosamine
-
 Saccharopolyspora rectivirgula
3.2.1.23 D-galactose
-
 Saccharopolyspora rectivirgula
3.2.1.23 EDTA
-
 Saccharopolyspora rectivirgula
3.2.1.23 EGTA
-
 Saccharopolyspora rectivirgula
3.2.1.23 isopropyl beta-D-thiogalactoside
-
 Saccharopolyspora rectivirgula
3.2.1.23 methyl beta-D-thiogalactoside
-
 Saccharopolyspora rectivirgula
3.2.1.23 Pb2+
-
 Saccharopolyspora rectivirgula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.04
-
 p-nitrophenyl-beta-D-galactoside
-
 Saccharopolyspora rectivirgula
3.2.1.23 0.75
-
 lactose
-
 Saccharopolyspora rectivirgula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.23 extracellular
-
 Saccharopolyspora rectivirgula
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Ca2+ 0.01 mM, activates Saccharopolyspora rectivirgula
3.2.1.23 K+ 10 mM, activates Saccharopolyspora rectivirgula
3.2.1.23 Mg2+ 0.01 mM, activates Saccharopolyspora rectivirgula
3.2.1.23 Mn2+ 0.01 mM, activates Saccharopolyspora rectivirgula
3.2.1.23 Na+ 10 mM, activates Saccharopolyspora rectivirgula
3.2.1.23 Na+ half-maximal activation at 0.1 mM Saccharopolyspora rectivirgula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 145000
-
 determination of sedimentation velocity and sedimention equilibrium Saccharopolyspora rectivirgula

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Saccharopolyspora rectivirgula
-
-
-
3.2.1.23 Saccharopolyspora rectivirgula V2-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Saccharopolyspora rectivirgula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharopolyspora rectivirgula 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharopolyspora rectivirgula V2-2 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose transgalactosylation Saccharopolyspora rectivirgula additional information oligosaccharides with the general structure (Gal)n-Glc, where n is 1, 2, 3, and 4 with a glucose at a reducing terminal ?
3.2.1.23 lactose transgalactosylation Saccharopolyspora rectivirgula V2-2 additional information oligosaccharides with the general structure (Gal)n-Glc, where n is 1, 2, 3, and 4 with a glucose at a reducing terminal ?
3.2.1.23 lactose + H2O
-
 Saccharopolyspora rectivirgula D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Saccharopolyspora rectivirgula V2-2 D-glucose + D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Saccharopolyspora rectivirgula p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Saccharopolyspora rectivirgula V2-2 p-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 monomer 1 * 145000, SDS-PAGE Saccharopolyspora rectivirgula

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 60
-
 pH 7.2, 4 h, in presence of 1.75 M lactose and 0.01 mM MnCl2, stable up to Saccharopolyspora rectivirgula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 47.6
-
 o-nitrophenyl-beta-D-galactoside
-
 Saccharopolyspora rectivirgula
3.2.1.23 55.8
-
 p-nitrophenyl-beta-D-galactoside
-
 Saccharopolyspora rectivirgula
3.2.1.23 63.1
-
 lactose
-
 Saccharopolyspora rectivirgula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.5 7.2 hydrolysis of lactose and p-nitrophenyl-beta-D-galactoside Saccharopolyspora rectivirgula