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Literature summary extracted from

  • Berger, J.L.; Lee, B.H.; Lacroix, C.
    Purification, properties and characterization of a high-molecular-mass beta-galactosidase isoenzyme from Thermus aquaticus (1997), Biotechnol. Appl. Biochem., 25, 29-41.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 2-mercaptoethanol activates Thermus aquaticus
3.2.1.23 Cys activates Thermus aquaticus
3.2.1.23 dithiothreitol activates Thermus aquaticus
3.2.1.23 glutathione activates Thermus aquaticus
3.2.1.23 Urea slight activation at 1 mM and at 10 mM Thermus aquaticus

General Stability

EC Number General Stability Organism
3.2.1.23 thermostability is enhanced by CaCl2 Thermus aquaticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Co2+ 2.5 mM CoCl2, inhibits hydrolysis of lactose and transferase activity Thermus aquaticus
3.2.1.23 EDTA 1 mM, 10% inhibition Thermus aquaticus
3.2.1.23 iodoacetate
-
Thermus aquaticus
3.2.1.23 p-chlorobenzene sulfonic acid
-
Thermus aquaticus
3.2.1.23 p-hydroxymercuribenzoate
-
Thermus aquaticus
3.2.1.23 phenylmethylsulfonyl fluoride 1 mM, 23% inhibition Thermus aquaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 6.59
-
o-nitrophenyl-beta-D-galactoside
-
Thermus aquaticus
3.2.1.23 36
-
lactose calculated from glucose liberated from lactose Thermus aquaticus
3.2.1.23 60
-
lactose calculated from disappearance from lactose Thermus aquaticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Ca2+ activates Thermus aquaticus
3.2.1.23 Co2+ activates Thermus aquaticus
3.2.1.23 Fe3+ activates Thermus aquaticus
3.2.1.23 K+ activates Thermus aquaticus
3.2.1.23 Li+ activates Thermus aquaticus
3.2.1.23 Mg2+ activates Thermus aquaticus
3.2.1.23 Na+ activates Thermus aquaticus
3.2.1.23 Zn2+ activates Thermus aquaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 59000
-
x * 59000, SDS-PAGE Thermus aquaticus
3.2.1.23 700000
-
gel filtration Thermus aquaticus
3.2.1.23 750000
-
non-denaturing PAGE Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Thermus aquaticus
-
-
-
3.2.1.23 Thermus aquaticus YT-I
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
Thermus aquaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 5.733
-
-
Thermus aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 1-naphthyl-beta-D-galactoside + H2O
-
Thermus aquaticus beta-D-galactose + 1-naphthol
-
?
3.2.1.23 1-naphthyl-beta-D-galactoside + H2O
-
Thermus aquaticus YT-I beta-D-galactose + 1-naphthol
-
?
3.2.1.23 1-naphthyl-beta-D-glucoside + H2O
-
Thermus aquaticus beta-D-glucose + 1-naphthol
-
?
3.2.1.23 1-naphthyl-beta-D-glucoside + H2O
-
Thermus aquaticus YT-I beta-D-glucose + 1-naphthol
-
?
3.2.1.23 lactose + arabinose transgalactosylation Thermus aquaticus ?
-
?
3.2.1.23 lactose + cellobiose transgalactosylation Thermus aquaticus ?
-
?
3.2.1.23 lactose + fructose transgalactosylation Thermus aquaticus ?
-
?
3.2.1.23 lactose + fructose transgalactosylation Thermus aquaticus YT-I ?
-
?
3.2.1.23 lactose + lactulose transgalactosylation Thermus aquaticus ?
-
?
3.2.1.23 lactose + lyxose transgalactosylation Thermus aquaticus ?
-
?
3.2.1.23 lactose + rhamnose transgalactosylation Thermus aquaticus ?
-
?
3.2.1.23 lactose + xylose transgalactosylation Thermus aquaticus ?
-
?
3.2.1.23 additional information specific for beta-D-anomeric linkages Thermus aquaticus ?
-
?
3.2.1.23 additional information specific for beta-D-anomeric linkages Thermus aquaticus YT-I ?
-
?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
Thermus aquaticus o-nitrophenol + beta-D-galactose
-
?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
Thermus aquaticus YT-I o-nitrophenol + beta-D-galactose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 ? x * 59000, SDS-PAGE Thermus aquaticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 80
-
at pH 7.0 Thermus aquaticus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 60 85 60°C: about 75% of maximal activity, 85°C: about 90% of maximal activity Thermus aquaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 additional information
-
thermostability is enhanced by CaCl2 Thermus aquaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 5.5
-
at 70°C Thermus aquaticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 4.5 6.5 about 45% of maximal activity at pH 4.5 and at pH 6.5 Thermus aquaticus