EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | 2-mercaptoethanol | activates | Thermus aquaticus | |
3.2.1.23 | Cys | activates | Thermus aquaticus | |
3.2.1.23 | dithiothreitol | activates | Thermus aquaticus | |
3.2.1.23 | glutathione | activates | Thermus aquaticus | |
3.2.1.23 | Urea | slight activation at 1 mM and at 10 mM | Thermus aquaticus |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.23 | thermostability is enhanced by CaCl2 | Thermus aquaticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | Co2+ | 2.5 mM CoCl2, inhibits hydrolysis of lactose and transferase activity | Thermus aquaticus | |
3.2.1.23 | EDTA | 1 mM, 10% inhibition | Thermus aquaticus | |
3.2.1.23 | iodoacetate | - |
Thermus aquaticus | |
3.2.1.23 | p-chlorobenzene sulfonic acid | - |
Thermus aquaticus | |
3.2.1.23 | p-hydroxymercuribenzoate | - |
Thermus aquaticus | |
3.2.1.23 | phenylmethylsulfonyl fluoride | 1 mM, 23% inhibition | Thermus aquaticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 6.59 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermus aquaticus | |
3.2.1.23 | 36 | - |
lactose | calculated from glucose liberated from lactose | Thermus aquaticus | |
3.2.1.23 | 60 | - |
lactose | calculated from disappearance from lactose | Thermus aquaticus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | Ca2+ | activates | Thermus aquaticus | |
3.2.1.23 | Co2+ | activates | Thermus aquaticus | |
3.2.1.23 | Fe3+ | activates | Thermus aquaticus | |
3.2.1.23 | K+ | activates | Thermus aquaticus | |
3.2.1.23 | Li+ | activates | Thermus aquaticus | |
3.2.1.23 | Mg2+ | activates | Thermus aquaticus | |
3.2.1.23 | Na+ | activates | Thermus aquaticus | |
3.2.1.23 | Zn2+ | activates | Thermus aquaticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 59000 | - |
x * 59000, SDS-PAGE | Thermus aquaticus |
3.2.1.23 | 700000 | - |
gel filtration | Thermus aquaticus |
3.2.1.23 | 750000 | - |
non-denaturing PAGE | Thermus aquaticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.23 | Thermus aquaticus | - |
- |
- |
3.2.1.23 | Thermus aquaticus YT-I | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.23 | - |
Thermus aquaticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 5.733 | - |
- |
Thermus aquaticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | 1-naphthyl-beta-D-galactoside + H2O | - |
Thermus aquaticus | beta-D-galactose + 1-naphthol | - |
? | |
3.2.1.23 | 1-naphthyl-beta-D-galactoside + H2O | - |
Thermus aquaticus YT-I | beta-D-galactose + 1-naphthol | - |
? | |
3.2.1.23 | 1-naphthyl-beta-D-glucoside + H2O | - |
Thermus aquaticus | beta-D-glucose + 1-naphthol | - |
? | |
3.2.1.23 | 1-naphthyl-beta-D-glucoside + H2O | - |
Thermus aquaticus YT-I | beta-D-glucose + 1-naphthol | - |
? | |
3.2.1.23 | lactose + arabinose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
3.2.1.23 | lactose + cellobiose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
3.2.1.23 | lactose + fructose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
3.2.1.23 | lactose + fructose | transgalactosylation | Thermus aquaticus YT-I | ? | - |
? | |
3.2.1.23 | lactose + lactulose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
3.2.1.23 | lactose + lyxose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
3.2.1.23 | lactose + rhamnose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
3.2.1.23 | lactose + xylose | transgalactosylation | Thermus aquaticus | ? | - |
? | |
3.2.1.23 | additional information | specific for beta-D-anomeric linkages | Thermus aquaticus | ? | - |
? | |
3.2.1.23 | additional information | specific for beta-D-anomeric linkages | Thermus aquaticus YT-I | ? | - |
? | |
3.2.1.23 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus aquaticus | o-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermus aquaticus YT-I | o-nitrophenol + beta-D-galactose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.23 | ? | x * 59000, SDS-PAGE | Thermus aquaticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 80 | - |
at pH 7.0 | Thermus aquaticus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 60 | 85 | 60°C: about 75% of maximal activity, 85°C: about 90% of maximal activity | Thermus aquaticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | additional information | - |
thermostability is enhanced by CaCl2 | Thermus aquaticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 5.5 | - |
at 70°C | Thermus aquaticus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 4.5 | 6.5 | about 45% of maximal activity at pH 4.5 and at pH 6.5 | Thermus aquaticus |