Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Onishi, N.; Tanaka, T.
    Purification and properties of a novel thermostable galactooligosaccharide-producing beta-galactosidase from Sterigmatomyces elviae CBS8119 (1995), Appl. Environ. Microbiol., 61, 4026-4030.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Hg2+
-
 Sterigmatomyces elviae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 2.4
-
 lactose
-
 Sterigmatomyces elviae
3.2.1.23 9.5
-
 o-nitrophenyl-beta-D-galactoside
-
 Sterigmatomyces elviae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 86000
-
 2 * 86000, SDS-PAGE Sterigmatomyces elviae
3.2.1.23 170000
-
 gel filtration Sterigmatomyces elviae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Sterigmatomyces elviae
-
-
-
3.2.1.23 Sterigmatomyces elviae CBS8119
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Sterigmatomyces elviae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 20.2
-
-
 Sterigmatomyces elviae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Sterigmatomyces elviae D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Sterigmatomyces elviae CBS8119 D-glucose + D-galactose
-
 ?
3.2.1.23 additional information high level of transgalactosylation activity Sterigmatomyces elviae ?
-
 ?
3.2.1.23 additional information high level of transgalactosylation activity Sterigmatomyces elviae CBS8119 ?
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Sterigmatomyces elviae o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Sterigmatomyces elviae CBS8119 o-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 dimer 2 * 86000, SDS-PAGE Sterigmatomyces elviae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 85
-
-
 Sterigmatomyces elviae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 80
-
 1 h, stable Sterigmatomyces elviae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4.5 5
-
 Sterigmatomyces elviae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 2.5 7 stable Sterigmatomyces elviae