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Literature summary extracted from

  • Leparoux, S.; Padrines, M.; Placier, G.; Colas, B.
    Characterization of a strictly specific acid beta-galactosidase from Achatina achatina (1997), Biochim. Biophys. Acta, 1336, 522-532.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.23 analysis enzyme can be used as a tool in the structural analysis of D-galactose-containing oligosaccharide chains, as well as for the synthesis of glycoconjugates Achatina achatina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 3.4
-
 2-nitrophenyl beta-D-galactoside
-
 Achatina achatina
3.2.1.23 6.7
-
 2-nitrophenyl beta-D-galactoside
-
 Achatina achatina
3.2.1.23 30
-
 lactose
-
 Achatina achatina

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Achatina achatina
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 glycoprotein contains 6% w/w carbohydrate, composed of rhamnose, fucose, xylose, mannose, galactose, glucose and N-acetylhexosamines Achatina achatina

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Achatina achatina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 digestive juice
-
 Achatina achatina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 10.2
-
-
 Achatina achatina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactoside + H2O
-
 Achatina achatina 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 lactose + 2-phenylethanol transgalactosylation Achatina achatina ?
-
 ?
3.2.1.23 lactose + benzyloxycarbonyl-Hyp-methyl ester transgalactosylation Achatina achatina ?
-
 ?
3.2.1.23 lactose + benzyloxycarbonyl-Ser-methyl ester transgalactosylation Achatina achatina ?
-
 ?
3.2.1.23 lactose + benzyloxycarbonyl-Thr-methyl ester transgalactosylation Achatina achatina ?
-
 ?
3.2.1.23 additional information cleaves beta1-4 linkage much faster than the beta1-3 and the beta1-6 linkage Achatina achatina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 monomer 1 * 120000-125000, SDS-PAGE Achatina achatina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 3.2
-
 hydrolysis Achatina achatina
3.2.1.23 5
-
 transgalactosylation Achatina achatina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 4 6.5 about 70% of maximal activity at pH 4.0 and at 6.5 Achatina achatina