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Literature summary extracted from

  • Tsumuraya, Y.; Mochizuki, N.; Hashimoto, Y.; Kovac, P.
    Purification of an exo-beta-(1->3)-D-galactanase of Irpex lacteus (Polyporus tulipiferae) and its action on arabinogalactan-proteins (1990), J. Biol. Chem., 265, 7207-7215.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 additional information
-
 additional information
-
 Irpex lacteus
3.2.1.23 19.9
-
 beta(1->3)-galactotriose
-
 Irpex lacteus
3.2.1.23 31.6
-
 beta-(1->3)-galactobiose
-
 Irpex lacteus
3.2.1.145 19.9
-
 beta-(1-3)-D-galactotriose
-
 Irpex lacteus
3.2.1.145 31.6
-
 beta-(1-3)-D-galactobiose
-
 Irpex lacteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 42000
-
 gel filtration Irpex lacteus
3.2.1.23 51000
-
 1 * 51000, denaturing PAGE Irpex lacteus
3.2.1.145 42000
-
 gel filtration Irpex lacteus
3.2.1.145 51000
-
 native PAGE Irpex lacteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.145 beta-(1-3)-D-galactan + H2O Irpex lacteus
-
 beta-(1-3)-D-galactan + D-galactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Irpex lacteus
-
 i.e. Polyporus tulipiferae
-
3.2.1.145 Irpex lacteus
-
 Polyporus tulipiferae
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 glycoprotein
-
 Irpex lacteus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Irpex lacteus
3.2.1.145 DEAE-cellulose, CM-Toyopearl, hydroylapatite, electrofocusing, purified from commercial driselase Irpex lacteus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.145 87.8
-
-
 Irpex lacteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 beta-(1->3)-D-galactan + H2O
-
 Irpex lacteus beta-D-galactose + ?
-
 ?
3.2.1.23 beta-1,3-galactobiose + H2O
-
 Irpex lacteus beta-D-galactose
-
 ?
3.2.1.23 beta-1,3-galactotriose + H2O
-
 Irpex lacteus ?
-
 ?
3.2.1.23 methyl beta-D-galactopentaoside + H2O
-
 Irpex lacteus ?
-
 ?
3.2.1.23 methyl beta-D-galactotetraoside + H2O
-
 Irpex lacteus ?
-
 ?
3.2.1.23 additional information specifically hydrolyzes oligosaccharides and polymers of (1-3)-linked beta-D-galactopyranosyl residues. Hydrolysis of carbohydrate portion of acacia gum and radish arabinogalactan-proteins increases with their decreasing branching. The action pattern of the enzyme includes the capability of bypassing the branching points. The side chains carrying additional D-galactosyl group at the reducing termini are released as neutral (1-6)-linked beta-D-galactooligosaccharides and their acidic derivatives having a 4-O-methyl-beta-D-glucuronosyl residue as the nonreducing end-group Irpex lacteus ?
-
 ?
3.2.1.145 beta-(1-3)-D-galactan + H2O
-
 Irpex lacteus beta-(1-3)-D-galactan + D-galactose
-
 ?
3.2.1.145 beta-(1-3)-D-galactotriose + H2O
-
 Irpex lacteus beta-(1-3)-D-galactobiose + D-galactose
-
 ?
3.2.1.145 beta-(1-3)-galactobiose + H2O
-
 Irpex lacteus D-galactose
-
 ?
3.2.1.145 beta-D-Gal-[(1->3)-beta-D-Gal]n + H2O only beta-(1, 3) oligomers act as substrates Irpex lacteus beta-D-Gal-[(1->3)-beta-D-Gal]n-1 + D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 monomer 1 * 51000, denaturing PAGE Irpex lacteus
3.2.1.145 monomer 1 * 51000, SDS-PAGE Irpex lacteus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.145 30 48 retains full activity up to 48°C, completely inactivated at 60°C Irpex lacteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 48
-
 pH 4.6, 10 min, stable up to Irpex lacteus
3.2.1.23 60
-
 pH 4.6, 10 min, complete inactivation Irpex lacteus
3.2.1.145 30 48 retains full activity up to 48°C, completely inactivated at 60°C Irpex lacteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4.6
-
 hydrolysis of beta-(1,3)-D-galactan Irpex lacteus
3.2.1.145 4.6
-
 enzyme action on beta-(1-3)-D-galactan Irpex lacteus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 3.5 7.5 4°C, 24 h, stable Irpex lacteus
3.2.1.145 3.5 7.5 after incubation for 24 h at 4°C Irpex lacteus