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Literature summary extracted from

  • Abe, M.; Higashi, S.
    beta-Glucosidase and beta-galactosidase from the periplasmic space of Rhizobium trifolii cells (1982), J. Gen. Appl. Microbiol., 28, 551-562.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Co2+ 50% inhibition at 8.5 mM Rhizobium leguminosarum
3.2.1.21 Cu2+ 98% inhibition at 1 mM Rhizobium leguminosarum
3.2.1.21 Fe2+ complete inhibition at 1 mM Rhizobium leguminosarum
3.2.1.21 Hg2+ 95% inhibition at 1 mM Rhizobium leguminosarum
3.2.1.21 p-chloromercuribenzoic acid complete inhibition at 0.085 mM Rhizobium leguminosarum
3.2.1.21 Zn2+ 84% inhibition at 1 mM Rhizobium leguminosarum
3.2.1.23 Cu2+
-
 Rhizobium leguminosarum
3.2.1.23 Fe2+
-
 Rhizobium leguminosarum
3.2.1.23 Hg2+
-
 Rhizobium leguminosarum
3.2.1.23 PCMB
-
 Rhizobium leguminosarum
3.2.1.23 Zn2+
-
 Rhizobium leguminosarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.003
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Rhizobium leguminosarum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 periplasmic space
-
 Rhizobium leguminosarum 42597
-
3.2.1.23 periplasmic space
-
 Rhizobium leguminosarum 42597
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 74000
-
 gel filtration Rhizobium leguminosarum
3.2.1.23 122000
-
 gel filtration Rhizobium leguminosarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Rhizobium leguminosarum sophorose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Rhizobium leguminosarum laminaribiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O Rhizobium leguminosarum gentiobiose beta-D-glucose + D-glucose
-
 ?
3.2.1.21 laminarin + H2O Rhizobium leguminosarum low activity beta-D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Rhizobium leguminosarum
-
-
-
3.2.1.23 Rhizobium leguminosarum
-
-
-
3.2.1.23 Rhizobium leguminosarum 4S
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 near homogeneity, chromatography techniques Rhizobium leguminosarum
3.2.1.23
-
 Rhizobium leguminosarum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 cell culture
-
 Rhizobium leguminosarum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Rhizobium leguminosarum 2-hydroxymethylphenol + beta-D-glucose
-
 ?
3.2.1.21 (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O low activity Rhizobium leguminosarum 4-hydroxyphenol + beta-D-glucopyranose
-
 ?
3.2.1.21 (4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Rhizobium leguminosarum 4-hydroxyphenol + beta-D-glucopyranose
-
 ?
3.2.1.21 (R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O amygdalin Rhizobium leguminosarum HCN + ?
-
 ?
3.2.1.21 2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Rhizobium leguminosarum beta-D-glucose + D-glucose
-
 ?
3.2.1.21 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Rhizobium leguminosarum beta-D-glucose + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rhizobium leguminosarum 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rhizobium leguminosarum 4S 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Rhizobium leguminosarum beta-D-glucose + D-glucose
-
 ?
3.2.1.21 beta-1,2-glucan + H2O low activity Rhizobium leguminosarum beta-D-glucose + ?
-
 ?
3.2.1.21 CM-cellulose + H2O low activity Rhizobium leguminosarum beta-D-glucose + ?
-
 ?
3.2.1.21 esculin + H2O
-
 Rhizobium leguminosarum beta-D-glucose + 6,7-dihydroxycoumarin
-
 ?
3.2.1.21 laminarin + H2O low activity Rhizobium leguminosarum beta-D-glucose + ?
-
 ?
3.2.1.21 luteose + H2O low activity Rhizobium leguminosarum beta-D-glucose + ?
-
 ?
3.2.1.21 methyl-beta-D-glucoside + H2O low activity Rhizobium leguminosarum beta-D-glucose + methanol
-
 ?
3.2.1.23 lactose + H2O
-
 Rhizobium leguminosarum D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Rhizobium leguminosarum 4S D-glucose + D-galactose
-
 ?
3.2.1.23 O-beta-D-galactosyl-1,3-D-arabinoside + H2O
-
 Rhizobium leguminosarum beta-D-galactose + D-arabinose
-
 ?
3.2.1.23 O-beta-D-galactosyl-1,3-D-arabinoside + H2O
-
 Rhizobium leguminosarum 4S beta-D-galactose + D-arabinose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-glucoside + H2O
-
 Rhizobium leguminosarum p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-glucoside + H2O
-
 Rhizobium leguminosarum 4S p-nitrophenol + beta-D-glucose
-
 ?
3.2.1.23 phenyl-beta-D-galactoside + H2O
-
 Rhizobium leguminosarum phenol + beta-D-galactose
-
 ?
3.2.1.23 phenyl-beta-D-galactoside + H2O
-
 Rhizobium leguminosarum 4S phenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 53
-
-
 Rhizobium leguminosarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 30 50 30°C: about 45% of maximal activity, 50°C: about 25% of maximal activity Rhizobium leguminosarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 50
-
 stable up to Rhizobium leguminosarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6.4
-
-
 Rhizobium leguminosarum
3.2.1.23 6.4
-
-
 Rhizobium leguminosarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 5 7.6 retains more than 80% of its activity Rhizobium leguminosarum
3.2.1.23 5.5 7.7 pH 5.5: about 50% of maximal activity, pH 7.7: about 30% of maximal activity Rhizobium leguminosarum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 6 7.2 rapid inactivation below pH 6.0 and above pH 7.2 Rhizobium leguminosarum