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Literature summary extracted from

  • Takahashi, T.; Sugahara, T.; Yamaya, S.
    Purification and characterization of a beta-galactosidase from Treponema phagedenis (Reiter strain) (1983), Curr. Microbiol., 8, 341-345.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cd2+
-
 Treponema phagedenis
3.2.1.23 Cu2+
-
 Treponema phagedenis
3.2.1.23 Hg2+
-
 Treponema phagedenis
3.2.1.23 PCMB
-
 Treponema phagedenis
3.2.1.23 Zn2+
-
 Treponema phagedenis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.29
-
 p-nitrophenyl-beta-D-galactoside
-
 Treponema phagedenis
3.2.1.23 0.36
-
 o-nitrophenyl-beta-D-galactoside
-
 Treponema phagedenis
3.2.1.23 5.4
-
 lactose
-
 Treponema phagedenis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 90000
-
 6 * 90000, SDS-PAGE Treponema phagedenis
3.2.1.23 580000
-
 gel filtration Treponema phagedenis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Treponema phagedenis
-
 Reiter strain
-
3.2.1.23 Treponema phagedenis Reiter
-
 Reiter strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Treponema phagedenis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 1126
-
-
 Treponema phagedenis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Treponema phagedenis D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Treponema phagedenis Reiter D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Treponema phagedenis o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Treponema phagedenis Reiter o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Treponema phagedenis p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Treponema phagedenis Reiter p-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 hexamer 6 * 90000, SDS-PAGE Treponema phagedenis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
 hydrolysis of p-nitrophenyl-beta-D-galactoside Treponema phagedenis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 35
-
 pH 6.5, 5 min, stable up to Treponema phagedenis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.5
-
 hydrolysis of p-nitrophenyl-beta-D-galactoside Treponema phagedenis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 6.5
-
 37°C, 1 h, stable only around Treponema phagedenis