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Literature summary extracted from

  • Takenishi, S.; Watanabe, Y.; Miwa, T.; Kobayashi, R.
    Purification and some properties of beta-galactosidase from Penicillium multicolor (1983), Agric. Biol. Chem., 47, 2533-2540.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cu2+
-
 Penicillium multicolor
3.2.1.23 Hg2+
-
 Penicillium multicolor
3.2.1.23 p-aminophenyl beta-D-thiogalactoside
-
 Penicillium multicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.6
-
 o-nitrophenyl-beta-D-galactoside
-
 Penicillium multicolor
3.2.1.23 8.9
-
 lactose
-
 Penicillium multicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 126000
-
 gel filtration Penicillium multicolor
3.2.1.23 130000
-
 1 * 130000, SDS-PAGE Penicillium multicolor

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Penicillium multicolor
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 glycoprotein 15% of the carbohydrate is mannose Penicillium multicolor

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Penicillium multicolor

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 145
-
-
 Penicillium multicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Penicillium multicolor D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Penicillium multicolor o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Penicillium multicolor p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 phenyl-beta-D-galactoside + H2O
-
 Penicillium multicolor phenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 monomer 1 * 130000, SDS-PAGE Penicillium multicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 60
-
-
 Penicillium multicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 30 70 30°C: about 40% of maximal activity, 70°C: about 30% of maximal activity Penicillium multicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 45
-
 pH 5.6, 15 min, retains more than 95% of its activity below Penicillium multicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4
-
-
 Penicillium multicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 3.5 7.5 40°C, 3 h, stable Penicillium multicolor
3.2.1.23 5.6
-
 room temperature, 10 days, retains more than 90% of maximal activity Penicillium multicolor