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Literature summary extracted from

  • Kelly, C.T.; O'Mahony, M.R.; Fogarty, W.M.
    A novel thermostable extracellular beta-galactosidase of Paecilomyces varioti (1988), Appl. Microbiol. Biotechnol., 27, 383-388.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 1-D-galactosylamine mixed-type inhibition Paecilomyces variotii
3.2.1.23 Ba2+ 10 mM, 28% inhibition Paecilomyces variotii
3.2.1.23 Cu2+ 10 mM, 28% inhibition Paecilomyces variotii
3.2.1.23 D-glucono-delta-lactone mixed-type inhibition Paecilomyces variotii
3.2.1.23 Fe2+ 10 mM, 20% inhibition Paecilomyces variotii
3.2.1.23 Hg2+ 10 mM, complete inactivation Paecilomyces variotii
3.2.1.23 Ni2+ 10 mM, 28% inhibition Paecilomyces variotii
3.2.1.23 p-hydroxymercuribenzoate 1 mM, complete inactivation Paecilomyces variotii
3.2.1.23 PCMB
-
 Paecilomyces variotii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 64
-
 lactose
-
 Paecilomyces variotii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.23 extracellular
-
 Paecilomyces variotii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 94000
-
 gel filtration Paecilomyces variotii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Paecilomyces variotii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.23 glycoprotein
-
 Paecilomyces variotii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Paecilomyces variotii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 additional information
-
-
 Paecilomyces variotii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 cellobiose + H2O 41.7% of the activity with lactose Paecilomyces variotii 2 D-glucose
-
 ?
3.2.1.23 gentiobiose + H2O 14.3% of the activity with lactose Paecilomyces variotii 2 beta-D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Paecilomyces variotii D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O weak activity Paecilomyces variotii o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-glucoside + H2O 20.6% of the activity with lactose Paecilomyces variotii p-nitrophenol + beta-D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
-
 Paecilomyces variotii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 50
-
 3 h, in presence of 4% w/v lactose, completely stable Paecilomyces variotii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 3.5
-
-
 Paecilomyces variotii