EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | 2-mercaptoethanol | 1 mM, activates | Thermoanaerobacter sp. | |
3.2.1.23 | Cys | 1 mM, activates | Thermoanaerobacter sp. | |
3.2.1.23 | dithiothreitol | 1 mM, activates | Thermoanaerobacter sp. | |
3.2.1.23 | EDTA | 1 mM, activates | Thermoanaerobacter sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | Ca2+ | 1.5 mM, 45% inhibition | Thermoanaerobacter sp. | |
3.2.1.23 | Co2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
3.2.1.23 | Cu2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
3.2.1.23 | Fe2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
3.2.1.23 | Fe3+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
3.2.1.23 | Mg2+ | 1.5 mM, 56% inhibition | Thermoanaerobacter sp. | |
3.2.1.23 | Ni2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
3.2.1.23 | PCMB | - |
Thermoanaerobacter sp. | |
3.2.1.23 | phenylmethylsulfonyl fluoride | - |
Thermoanaerobacter sp. | |
3.2.1.23 | Zn2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 4.3 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermoanaerobacter sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.23 | intracellular | - |
Thermoanaerobacter sp. | 5622 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.23 | K+ | 0.1 M, activates | Thermoanaerobacter sp. | |
3.2.1.23 | Li+ | 0.1 M, activates | Thermoanaerobacter sp. | |
3.2.1.23 | Na+ | 0.1 M, activates | Thermoanaerobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 154000 | - |
gel filtration | Thermoanaerobacter sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.23 | Thermoanaerobacter sp. | - |
- |
- |
3.2.1.23 | Thermoanaerobacter sp. TP6-B1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.23 | - |
Thermoanaerobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 99 | - |
at 70°C | Thermoanaerobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.23 | lactose + H2O | - |
Thermoanaerobacter sp. | D-glucose + D-galactose | - |
? | |
3.2.1.23 | lactose + H2O | - |
Thermoanaerobacter sp. TP6-B1 | D-glucose + D-galactose | - |
? | |
3.2.1.23 | methyl O-beta-galactoside + H2O | - |
Thermoanaerobacter sp. | methanol + beta-D-galactose | - |
? | |
3.2.1.23 | methyl O-beta-galactoside + H2O | - |
Thermoanaerobacter sp. TP6-B1 | methanol + beta-D-galactose | - |
? | |
3.2.1.23 | methylthio-beta-D-galactoside + H2O | - |
Thermoanaerobacter sp. | galactose + methanethiol | - |
? | |
3.2.1.23 | methylthio-beta-D-galactoside + H2O | - |
Thermoanaerobacter sp. TP6-B1 | galactose + methanethiol | - |
? | |
3.2.1.23 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermoanaerobacter sp. | o-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermoanaerobacter sp. TP6-B1 | o-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.23 | p-nitrophenyl alpha-L-arabinoside + H2O | - |
Thermoanaerobacter sp. | p-nitrophenol + alpha-L-arabinose | - |
? | |
3.2.1.23 | p-nitrophenyl alpha-L-arabinoside + H2O | - |
Thermoanaerobacter sp. TP6-B1 | p-nitrophenol + alpha-L-arabinose | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 70 | - |
half-life: 220 min | Thermoanaerobacter sp. |
3.2.1.23 | 75 | - |
half-life: 155 min | Thermoanaerobacter sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.23 | 431 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermoanaerobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 6 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside | Thermoanaerobacter sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.23 | 5.2 | 7.6 | more than 50% of maximal activity at pH 5.2 and at pH 7.6 | Thermoanaerobacter sp. |