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Literature summary extracted from

  • Wallenfels, K.; Malhotra, O.M.
    beta-Galactosidase (1960), The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 4, 409-430.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.23 4 M urea, irreversible inactivation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ag+
-
 Aspergillus oryzae
3.2.1.23 Ag+
-
 Escherichia coli
3.2.1.23 Cd2+
-
 Escherichia coli
3.2.1.23 Cu2+
-
 Aspergillus oryzae
3.2.1.23 Cu2+
-
 Escherichia coli
3.2.1.23 Cu2+
-
 Saccharomyces fragilis
3.2.1.23 Hg2+
-
 Aspergillus oryzae
3.2.1.23 Hg2+
-
 Escherichia coli
3.2.1.23 Hg2+
-
 Felis catus
3.2.1.23 Hg2+
-
 Medicago sativa
3.2.1.23 Hg2+
-
 Saccharomyces fragilis
3.2.1.23 Pb2+
-
 Escherichia coli
3.2.1.23 PCMB
-
 Escherichia coli
3.2.1.23 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 additional information
-
 additional information
-
 Homo sapiens
3.2.1.23 additional information
-
 additional information influence of temperature on Km-value of o-nitrophenyl-beta-D-galactoside Escherichia coli
3.2.1.23 0.0513
-
 p-nitrophenyl-beta-D-galactoside with 0.05 M NaCl Escherichia coli
3.2.1.23 0.12
-
 thio-(o-nitrophenyl)-beta-D-galactoside
-
 Escherichia coli
3.2.1.23 0.161
-
 o-nitrophenyl-beta-D-galactoside with 0.05 M NaCl Escherichia coli
3.2.1.23 0.445
-
 p-nitrophenyl-beta-D-galactoside without NaCl Escherichia coli
3.2.1.23 0.95
-
 o-nitrophenyl-beta-D-galactoside without NaCl Escherichia coli
3.2.1.23 1.47
-
 phenyl-beta-D-galactoside with 0.05 M NaCl Escherichia coli
3.2.1.23 1.9
-
 alpha-lactose with 0.05 M NaCl Escherichia coli
3.2.1.23 2.5
-
 methyl salicylate beta-D-galactoside
-
 Escherichia coli
3.2.1.23 3.23
-
 phenyl-beta-D-galactoside without NaCl Escherichia coli
3.2.1.23 7.52
-
 alpha-lactose without NaCl Escherichia coli
3.2.1.23 8.3
-
 allolactose without NaCl Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+
-
 Medicago sativa
3.2.1.23 K+
-
 Saccharomyces fragilis
3.2.1.23 K+ activates hydrolysis of lactose more effectively than Na+ Escherichia coli
3.2.1.23 Mg2+
-
 Medicago sativa
3.2.1.23 Mg2+
-
 Saccharomyces fragilis
3.2.1.23 Mg2+ strain ML 308 shows absolute requirement, strain ML 309 does not Escherichia coli
3.2.1.23 Na+
-
 Medicago sativa
3.2.1.23 Na+
-
 Felis catus
3.2.1.23 Na+ activates hydrolysis of o-nitrophenyl-beta-D-galactoside more effectively than K+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Aspergillus flavus
-
-
-
3.2.1.23 Aspergillus niger
-
-
-
3.2.1.23 Aspergillus oryzae
-
-
-
3.2.1.23 Bos taurus
-
-
-
3.2.1.23 Canis lupus familiaris
-
-
-
3.2.1.23 Capra hircus
-
-
-
3.2.1.23 Coffea sp.
-
 coffee
-
3.2.1.23 Escherichia coli
-
-
-
3.2.1.23 Escherichia coli K 12
-
-
-
3.2.1.23 Escherichia coli ML 308
-
-
-
3.2.1.23 Escherichia coli ML 309
-
-
-
3.2.1.23 Felis catus
-
-
-
3.2.1.23 Helix pomatia
-
-
-
3.2.1.23 Homo sapiens
-
-
-
3.2.1.23 Klebsiella aerogenes
-
-
-
3.2.1.23 Malus sp.
-
-
-
3.2.1.23 Medicago sativa
-
-
-
3.2.1.23 Neurospora sp.
-
-
-
3.2.1.23 Oryctolagus cuniculus
-
-
-
3.2.1.23 Ovis aries
-
-
-
3.2.1.23 Prunus armeniaca
-
-
-
3.2.1.23 Prunus dulcis
-
 almond
-
3.2.1.23 Prunus persica
-
-
-
3.2.1.23 Saccharomyces fragilis
-
-
-
3.2.1.23 Shigella sonnei
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.23 beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose + 3 H2O = 4 beta-D-galactopyranose mechanism Escherichia coli
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 intestinal juice
-
 Homo sapiens
-
3.2.1.23 intestine
-
 Homo sapiens
-
3.2.1.23 intestine mucous membrane Bos taurus
-
3.2.1.23 mammary gland
-
 Bos taurus
-
3.2.1.23 mammary gland
-
 Felis catus
-
3.2.1.23 mammary gland
-
 Capra hircus
-
3.2.1.23 saliva
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 allolactose + H2O
-
 Escherichia coli D-galactose + D-glucose
-
 ?
3.2.1.23 allolactose + H2O
-
 Escherichia coli K 12 D-galactose + D-glucose
-
 ?
3.2.1.23 allolactose + H2O
-
 Escherichia coli ML 308 D-galactose + D-glucose
-
 ?
3.2.1.23 allolactose + H2O
-
 Escherichia coli ML 309 D-galactose + D-glucose
-
 ?
3.2.1.23 alpha-lactose + H2O
-
 Escherichia coli D-galactose + D-glucose
-
 ?
3.2.1.23 lactose + H2O
-
 Homo sapiens D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Aspergillus niger D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Aspergillus oryzae D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O alpha-lactose Escherichia coli D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O alpha-lactose Escherichia coli K 12 D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O alpha-lactose Escherichia coli ML 308 D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O alpha-lactose Escherichia coli ML 309 D-glucose + D-galactose
-
 ?
3.2.1.23 methyl salicylate beta-D-galactoside + H2O
-
 Escherichia coli methyl salicylate + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Escherichia coli o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Homo sapiens o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Bos taurus o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus niger o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Medicago sativa o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Saccharomyces fragilis o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus oryzae o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Escherichia coli K 12 o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Escherichia coli ML 308 o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Escherichia coli ML 309 o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Escherichia coli p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Homo sapiens p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Bos taurus p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus niger p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Felis catus p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Escherichia coli K 12 p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Escherichia coli ML 308 p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl-beta-D-galactoside + H2O
-
 Escherichia coli ML 309 p-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 phenyl-beta-D-galactoside + H2O
-
 Escherichia coli phenol + beta-D-galactose
-
 ?
3.2.1.23 phenyl-beta-D-galactoside + H2O
-
 Escherichia coli K 12 phenol + beta-D-galactose
-
 ?
3.2.1.23 phenyl-beta-D-galactoside + H2O
-
 Escherichia coli ML 308 phenol + beta-D-galactose
-
 ?
3.2.1.23 phenyl-beta-D-galactoside + H2O
-
 Escherichia coli ML 309 phenol + beta-D-galactose
-
 ?
3.2.1.23 thio-(o-nitrophenyl)-beta-D-galactoside + H2O
-
 Escherichia coli thio-(o-nitrophenol) + beta-D-galactose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 6.6
-
 in presence of 0.05 M NaCl, strain ML 309 Escherichia coli
3.2.1.23 7.2 7.3 30°C, phosphate buffer, strain K-12 Escherichia coli
3.2.1.23 7.7
-
 20°C, 0.05 M Tris-acetate buffer, strain ML 309 Escherichia coli