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Literature summary extracted from

  • Zeilinger, S.; Kristufek, D.; Arisan-Atac, I.; Hodits, R.; Kubicek, C.P.
    Conditions of formation, purification, and characterization of an alpha-galactosidase of Trichoderma reesei RUT C-30 (1993), Appl. Biochem. Biotechnol., 59, 1347-1353.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 1.2
-
 p-nitrophenyl alpha-D-galactoside
-
 Trichoderma reesei
3.2.1.22 1.3
-
 melibiose
-
 Trichoderma reesei
3.2.1.22 1.8
-
 stachyose
-
 Trichoderma reesei
3.2.1.22 3.8
-
 raffinose
-
 Trichoderma reesei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.22 extracellular
-
 Trichoderma reesei
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 50000
-
 gel filtration Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Trichoderma reesei
-
 RUT C-30
-
3.2.1.22 Trichoderma reesei RUT C-30
-
 RUT C-30
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Trichoderma reesei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 30
-
-
 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O
-
 Trichoderma reesei D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Trichoderma reesei RUT C-30 D-galactose + D-glucose
-
 ?
3.2.1.22 p-nitrophenyl-alpha-D-galactoside + H2O
-
 Trichoderma reesei p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl-alpha-D-galactoside + H2O
-
 Trichoderma reesei RUT C-30 p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Trichoderma reesei sucrose + D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Trichoderma reesei RUT C-30 sucrose + D-galactose
-
 ?
3.2.1.22 stachyose + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.22 stachyose + H2O
-
 Trichoderma reesei RUT C-30 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 monomer
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 60
-
-
 Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 50
-
 1 h, 70% of activity recovered Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 4
-
-
 Trichoderma reesei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 4 8
-
 Trichoderma reesei