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Literature summary extracted from

  • Fridjonsson, O.; Watzlawick, H.; Gehweiler, A.; Mattes, R.
    Thermostable alpha-Galactosidas from Bacillus stearothermophilus NUB3621: cloning, sequencing and characterization (1999), FEMS Microbiol. Lett., 176, 147-153.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expression in Escherichia coli Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.38
-
p-nitrophenyl alpha-D-galactoside
-
Geobacillus stearothermophilus
3.2.1.22 12
-
melibiose
-
Geobacillus stearothermophilus
3.2.1.22 17.4
-
raffinose
-
Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 80300
-
4 * 80300 Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Geobacillus stearothermophilus
-
NUB3621
-
3.2.1.22 Geobacillus stearothermophilus NUB3621
-
NUB3621
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O
-
Geobacillus stearothermophilus D-galactose + D-glucose
-
?
3.2.1.22 melibiose + H2O
-
Geobacillus stearothermophilus NUB3621 D-galactose + D-glucose
-
?
3.2.1.22 raffinose + H2O
-
Geobacillus stearothermophilus sucrose + D-galactose
-
?
3.2.1.22 raffinose + H2O
-
Geobacillus stearothermophilus NUB3621 sucrose + D-galactose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 tetramer 4 * 80300 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 75
-
-
Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 70
-
19 h, 50% loss in activity Geobacillus stearothermophilus