Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Roggentin, P.; Kleineidam, R.G.; Schauer, R.
    Diversity in the properties of two sialidase isoenzymes produced by Clostridium perfringens spp. (1995), Biol. Chem. Hoppe-Seyler, 376, 569-575.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 expression in Escherichia coli Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.18 0.08
-
 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid DSM 756, large salidase and A99, large salidase Clostridium perfringens
3.2.1.18 0.18
-
 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid A99, small salidase, cloned Clostridium perfringens
3.2.1.18 0.19
-
 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid A99, small salidase Clostridium perfringens
3.2.1.18 0.36
-
 poly-alpha-2,8-lactose A99, large salidase Clostridium perfringens
3.2.1.18 0.5
-
 poly-alpha-2,8-lactose DSM 756, large salidase Clostridium perfringens
3.2.1.18 0.53
-
 sialyl-alpha-2,3-lactose A99, small salidase Clostridium perfringens
3.2.1.18 0.56
-
 sialyl-alpha-2,3-lactose A99, large salidase Clostridium perfringens
3.2.1.18 0.58
-
 sialyl-alpha-2,3-lactose DSM 756, large salidase Clostridium perfringens
3.2.1.18 1.7
-
 sialyl-alpha-2,6-lactose A99, large salidase Clostridium perfringens
3.2.1.18 1.9
-
 sialyl-alpha-2,3-lactose A99, small salidase, cloned Clostridium perfringens
3.2.1.18 2.2
-
 sialyl-alpha-2,6-lactose DSM 756, large salidase Clostridium perfringens
3.2.1.18 2.8
-
 sialyl-alpha-2,6-lactose A99, small salidase, cloned Clostridium perfringens
3.2.1.18 3.5
-
 sialyl-alpha-2,6-lactose A99, small salidase Clostridium perfringens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.18 cell associated
-
 Clostridium perfringens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.18 32000
-
 gel filtration, small sialidase Clostridium perfringens
3.2.1.18 63000
-
 gel filtration, higher molecular mass sialidase Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Clostridium perfringens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18
-
 Clostridium perfringens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.18 1.03
-
 A99, small sialidase Clostridium perfringens
3.2.1.18 390
-
 A99, large sialidase Clostridium perfringens
3.2.1.18 570
-
 DSM 756, large sialidase Clostridium perfringens
3.2.1.18 740
-
 A99, small sialidase, cloned Clostridium perfringens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 poly-alpha-2,8-lactose + H2O
-
 Clostridium perfringens ?
-
 ?
3.2.1.18 sialyl-alpha-2,3-lactose + H2O
-
 Clostridium perfringens ?
-
 ?
3.2.1.18 sialyl-alpha-2,6-lactose + H2O
-
 Clostridium perfringens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.18 monomer
-
 Clostridium perfringens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.18 37
-
 small sialidase Clostridium perfringens
3.2.1.18 51 55 higher molecular mass sialidase Clostridium perfringens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.18 5 55 small sialidase Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.18 5.5
-
 higher molecular mass sialidase Clostridium perfringens
3.2.1.18 6.1
-
 small sialidase Clostridium perfringens