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Literature summary extracted from

  • Wang, S.L.; Chang, W.T.
    Purification and characterization of two bifunctional chitinases/lysozymes extracellulary produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium (1997), Appl. Environ. Microbiol., 63, 380-386.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.17 2-mercaptoethanol
-
 Pseudomonas aeruginosa
3.2.1.17 dithiothreitol
-
 Pseudomonas aeruginosa
3.2.1.17 glutathione
-
 Pseudomonas aeruginosa
3.2.1.17 MgCl2
-
 Pseudomonas aeruginosa
3.2.1.17 Mn2+
-
 Pseudomonas aeruginosa
3.2.1.17 ZnCl2
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.17 Cu2+ activates Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.17 30000
-
 FII, gel filtration Pseudomonas aeruginosa
3.2.1.17 32000
-
 FII, 1 * 32000, SDS-PAGE Pseudomonas aeruginosa
3.2.1.17 60000
-
 FI, gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.17 Pseudomonas aeruginosa
-
 K-187, two extracellular forms FI and FII
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.17 0.085
-
 FII Pseudomonas aeruginosa
3.2.1.17 0.103
-
 FI Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.17 colloidal chitin
-
 Pseudomonas aeruginosa sugars
-
 ?
3.2.1.17 ethylene glycol chitin + H2O
-
 Pseudomonas aeruginosa sugars
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.17 dimer 2 * 30000, SDS-PAGE Pseudomonas aeruginosa
3.2.1.17 monomer FII, 1 * 32000, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.17 40
-
 FII Pseudomonas aeruginosa
3.2.1.17 50
-
 FI Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.17 50
-
 FI unaffected, 10 min Pseudomonas aeruginosa
3.2.1.17 60
-
 FII, unaffected, 10 min Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.17 7
-
 FII Pseudomonas aeruginosa
3.2.1.17 8
-
 FI Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.17 5 10 FII Pseudomonas aeruginosa
3.2.1.17 6 9 FI Pseudomonas aeruginosa