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Literature summary extracted from

  • Mukherjee, J.J.; Jay, F.T.; Choy, P.C.
    Purification, characterization and modulation of a microsomal carboxylesterase in rat liver for the hydrolysis of acyl-CoA (1993), Biochem. J., 295, 81-86.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.1 lysophosphatidylcholine activate Rattus norvegicus
3.1.1.1 phosphatidylcholine activates Rattus norvegicus
3.1.1.1 phosphatidylethanolamine activates Rattus norvegicus
3.1.2.2 lysophosphatidylcholine
-
 Rattus norvegicus
3.1.2.2 lysophosphatidylethanolamine
-
 Rattus norvegicus
3.1.2.2 phosphatidylcholine
-
 Rattus norvegicus
3.1.2.2 phosphatidylethanolamine
-
 Rattus norvegicus
3.1.2.20 lysophosphatidylcholine
-
 Rattus norvegicus
3.1.2.20 lysophosphatidylethanolamine
-
 Rattus norvegicus
3.1.2.20 phosphatidylcholine
-
 Rattus norvegicus
3.1.2.20 phosphatidylethanolamine
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 lysophosphatidic acid
-
 Rattus norvegicus
3.1.2.2 cardiolipin
-
 Rattus norvegicus
3.1.2.2 lysophosphatidic acid
-
 Rattus norvegicus
3.1.2.20 cardiolipin
-
 Rattus norvegicus
3.1.2.20 lysophosphatidic acid
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.017
-
 palmitoyl-CoA
-
 Rattus norvegicus
3.1.2.2 0.017
-
 hexadecanoyl-CoA
-
 Rattus norvegicus
3.1.2.20 0.017
-
 hexadecanoyl-CoA
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 microsome
-
 Rattus norvegicus
-
-
3.1.2.2 microsome
-
 Rattus norvegicus
-
-
3.1.2.20 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 56000
-
 1 * 56000, SDS-PAGE Rattus norvegicus
3.1.1.1 64000
-
 gel filtration Rattus norvegicus
3.1.2.2 56000
-
 x * 56000, SDS-PAGE Rattus norvegicus
3.1.2.2 64000
-
 gel filtration Rattus norvegicus
3.1.2.20 56000
-
 x * 56000, SDS-PAGE Rattus norvegicus
3.1.2.20 64000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Rattus norvegicus
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Rattus norvegicus
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Rattus norvegicus
-
-
-
3.1.2.2 Rattus norvegicus
-
-
-
3.1.2.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.1 liver
-
 Rattus norvegicus
-
3.1.2.2 liver
-
 Rattus norvegicus
-
3.1.2.20 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 3.1
-
 hydrolysis of palmitoyl-CoA Rattus norvegicus
3.1.1.1 66.7
-
 hydrolysis of 4-nitrophenyl acetate Rattus norvegicus
3.1.2.2 3.1
-
-
 Rattus norvegicus
3.1.2.2 66.7
-
-
 Rattus norvegicus
3.1.2.20 3.1
-
-
 Rattus norvegicus
3.1.2.20 66.7
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -70°C in the presence of 20% glycerol over 1 month Rattus norvegicus
3.1.2.20 -70°C in the presence of 20% glycerol over 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Rattus norvegicus 4-nitrophenol + acetate
-
 ?
3.1.1.1 oleoyl-CoA + H2O
-
 Rattus norvegicus oleic acid + CoA
-
 ?
3.1.1.1 palmitoyl-CoA + H2O
-
 Rattus norvegicus palmitic acid + CoA
-
 ?
3.1.1.1 stearoyl-CoA + H2O
-
 Rattus norvegicus CoA + stearate
-
 ?
3.1.2.2 4-nitrophenyl acetate + H2O
-
 Rattus norvegicus 4-nitrophenol + acetate
-
 ?
3.1.2.2 acyl-CoA + H2O long-chain fatty-acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Rattus norvegicus CoA + octadecanoate
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Rattus norvegicus CoA + oleate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Rattus norvegicus CoA + palmitate
-
 ?
3.1.2.20 4-nitrophenyl acetate + H2O
-
 Rattus norvegicus 4-nitrophenol + acetate
-
 ?
3.1.2.20 acyl-CoA + H2O long-chain fatty-acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Rattus norvegicus CoA + octadecanoate
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Rattus norvegicus CoA + oleate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Rattus norvegicus CoA + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 56000, SDS-PAGE Rattus norvegicus
3.1.2.2 ? x * 56000, SDS-PAGE Rattus norvegicus
3.1.2.20 ? x * 56000, SDS-PAGE Rattus norvegicus