Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pindel, E.V.; Kedishvili, N.Y.; Abraham, T.L.; Brzezinski, M.R.; Zhang, J.; Dean, R.A.; Bosron, W.F.
    Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin (1997), J. Biol. Chem., 272, 14769-14775.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.84
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.84 eserine hCE-2 shows greater inhibition by eserine thann hCE-1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.84 0.13
-
 6-monoacetylmorphine pH 7.4, 37°C Homo sapiens
3.1.1.84 0.15
-
 4-yethylumbelliferyl acetate pH 7.4, 37°C Homo sapiens
3.1.1.84 0.39
-
 cocaine pH 7.4, 37°C Homo sapiens
3.1.1.84 6.8
-
 heroin pH 7.4, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.84 60000
-
 1 * 60000, SDS-PAGE Homo sapiens
3.1.1.84 80000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.84 cocaine + H2O Homo sapiens hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine. hCE-2 may play an important role in the degradation of cocaine and heroin in human tissues ecgonine methyl ester + benzoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.84 Homo sapiens O00748
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.84 glycoprotein high mannose type Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.84
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.84 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.84 4-methylumbelliferyl acetate + H2O hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1 Homo sapiens 4-methylumbelliferone + acetate
-
 ?
3.1.1.84 6-monoacetylmorphine + H2O hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1 Homo sapiens morphine + acetate
-
 ?
3.1.1.84 cocaine + H2O hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine. hCE-2 may play an important role in the degradation of cocaine and heroin in human tissues Homo sapiens ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine Homo sapiens ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 heroin + H2O hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1 Homo sapiens 6-monoacetylmorphine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.84 monomer 1 * 60000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.84 hCE-2
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.84 0.0001
-
 eserine
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.84 Homo sapiens isoelectric focusing
-
 4.9