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Literature summary extracted from

  • Dudman, N.P.B.; Zerner, B.
    Carboxylesterase from pig and ox liver (1975), Methods Enzymol., 35B, 190-208.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.1
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 206000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Bos taurus
-
-
-
3.1.1.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Sus scrofa
3.1.1.1
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.1 liver
-
 Sus scrofa
-
3.1.1.1 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.1.1.1 -15°C, 0.02 M potassium phosphate, pH 7.4, stable for 1.5 years Sus scrofa
3.1.1.1 4°C, suspension in (NH4)2SO4 solution, stable for 8 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 2,4-dinitrophenyl butyrate + H2O
-
 Sus scrofa 2,4-dinitrophenol + butyrate
-
 ?
3.1.1.1 2-nitrophenyl butyrate + H2O
-
 Sus scrofa 2-nitrophenol + butyrate
-
 ?
3.1.1.1 3-nitrophenyl butyrate + H2O
-
 Sus scrofa 3-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Sus scrofa 4-nitrophenol + butyrate
-
 ?
3.1.1.1 benzyloxycarbonyl-D-Tyr-p-nitrophenyl ester + H2O
-
 Sus scrofa benzyloxycarbonyl-D-Tyr + 4-nitrophenol
-
 ?
3.1.1.1 benzyloxycarbonyl-L-Tyr-p-nitrophenyl ester + H2O
-
 Sus scrofa benzyloxycarbonyl-L-Tyr + 4-nitrophenol
-
 ?
3.1.1.1 D-Tyr ethyl ester + H2O
-
 Sus scrofa D-Tyr + ethanol
-
 ?
3.1.1.1 ethyl butyrate + H2O
-
 Sus scrofa ethanol + butyrate
-
 ?
3.1.1.1 L-Tyr ethyl ester + H2O
-
 Sus scrofa L-Tyr + ethanol
-
 ?
3.1.1.1 phenyl acetate + H2O
-
 Sus scrofa phenol + acetate
-
 ?
3.1.1.1 phenyl acetate + H2O
-
 Bos taurus phenol + acetate
-
 ?
3.1.1.1 phenyl butyrate + H2O
-
 Sus scrofa phenol + butyrate
-
 ?
3.1.1.1 phenyl butyrate + H2O
-
 Bos taurus phenol + butyrate
-
 ?
3.1.1.1 phenyl formate + H2O
-
 Sus scrofa phenol + formate
-
 ?
3.1.1.1 phenyl pentanoate + H2O
-
 Sus scrofa phenol + pentanoate
-
 ?
3.1.1.1 phenyl pivalate + H2O
-
 Bos taurus phenol + pivalate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 additional information
-
 additional information
-
 Bos taurus
3.1.1.1 0.9
-
 benzyloxycarbonyl-D-Tyr p-nitrophenyl ester
-
 Sus scrofa
3.1.1.1 6.5
-
 phenyl trimethyl acetate
-
 Bos taurus
3.1.1.1 24
-
 benzyloxycarbonyl-L-Tyr p-nitrophenyl ester
-
 Sus scrofa
3.1.1.1 71
-
 L-Tyr ethyl ester
-
 Sus scrofa
3.1.1.1 71
-
 D-Tyr ethyl ester
-
 Sus scrofa
3.1.1.1 233
-
 2,4-dinitrophenyl butyrate
-
 Sus scrofa
3.1.1.1 300
-
 phenyl acetate
-
 Bos taurus
3.1.1.1 345
-
 3-nitrophenyl butyrate
-
 Sus scrofa
3.1.1.1 348
-
 o-nitrophenyl butyrate
-
 Sus scrofa
3.1.1.1 367
-
 p-nitrophenyl butyrate
-
 Sus scrofa
3.1.1.1 380
-
 phenyl acetate
-
 Sus scrofa
3.1.1.1 519
-
 phenyl butyrate
-
 Sus scrofa
3.1.1.1 551
-
 ethyl butyrate
-
 Sus scrofa
3.1.1.1 660
-
 phenyl formate
-
 Sus scrofa
3.1.1.1 860
-
 phenyl pentanoate
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.1 2.3
-
 20°C, 9 h, complete loss of activity Sus scrofa
3.1.1.1 4.5
-
 4°C, 8 h, 30% loss of activity, rapid dissociation to monomeric units Sus scrofa
3.1.1.1 5
-
 7% loss of activity after 7 months Sus scrofa