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Literature summary extracted from

  • Krell, H.W.; Sandermann, H.
    Plant biochemistry of xenobiotics. Purification and properties of a wheat esterase hydrolyzing the plasticizer chemical, bis(2-ethylhexyl)phthalate (1984), Eur. J. Biochem., 143, 57-62.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.60
-
 Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 4-mercuribenzoate complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
3.1.1.1 bis(4-nitrophenyl) phosphate 25% inhibition at 1 mM, no inhibition at 0.1 mM Triticum aestivum
3.1.1.1 DFP complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
3.1.1.1 eserine complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
3.1.1.1 Hg2+ complete inhibition at 1 mM, 80% inhibition at 0.1 mM; HgCl2 Triticum aestivum
3.1.1.1 mono-4-nitrophenyl phosphoric acid ester 25% inhibition at 1 mM, no inhibition at 0.1 mM Triticum aestivum
3.1.1.1 paraoxon 0.1 mM and 1 mM, complete inhibition Triticum aestivum
3.1.1.1 PMSF 75% inhibition at 1 mM, 10% inhibition at 0.1 mM Triticum aestivum
3.1.1.60 4-mercuribenzoate complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
3.1.1.60 4-nitrophenyl phosphate
-
 Triticum aestivum
3.1.1.60 4-nitrophenyl-diethyl phosphate synonym: Paraoxon, complete inhibition at 0.1 and 1 mM Triticum aestivum
3.1.1.60 cholate
-
 Triticum aestivum
3.1.1.60 deoxycholate
-
 Triticum aestivum
3.1.1.60 diisopropylfluorophosphate complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
3.1.1.60 Dimethylsulfoxide 70% inihibition at 5% Triticum aestivum
3.1.1.60 dodecyl beta-maltoside
-
 Triticum aestivum
3.1.1.60 eserine complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
3.1.1.60 ethyleneglycolmonomethyl ether 70% inihibition at 5% Triticum aestivum
3.1.1.60 HgCl2 complete inhibition at 1 mM, 80% inhibition at 0.1 mM Triticum aestivum
3.1.1.60 Lubrol Px
-
 Triticum aestivum
3.1.1.60 phenylmethylsulfonyl fluoride 75% inhibition at 1 mM Triticum aestivum
3.1.1.60 Span-20
-
 Triticum aestivum
3.1.1.60 taurocholate
-
 Triticum aestivum
3.1.1.60 Triton X-100
-
 Triticum aestivum
3.1.1.60 Tween-20
-
 Triticum aestivum
3.1.1.60 Tween-80
-
 Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.005
-
 bis(2-ethylhexyl)phthalate
-
 Triticum aestivum
3.1.1.60 0.005
-
 bis(2-ethylhexyl)phthalate 30°C Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 22000
-
 x * 22000, SDS-PAGE Triticum aestivum
3.1.1.1 38000
-
 gel filtration Triticum aestivum
3.1.1.60 22000
-
 x * 22000, SDS-PAGE Triticum aestivum
3.1.1.60 38000
-
 gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.60 bis(2-ethylhexyl)phthalate + H2O Triticum aestivum
-
 2-ethylhexyl phthalate + 2-ethylhexan-1-ol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Triticum aestivum
-
-
-
3.1.1.60 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.60 leaf
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.60 0.00077
-
-
 Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
3.1.1.1 -70°C, stable for several weeks Triticum aestivum
3.1.1.1 4°C, half-life: about 1 weeks Triticum aestivum
3.1.1.60 4°C, half-life: 1 week for the enzyme from first steps of purification, 1 day for the enzyme from last steps of purification and purified enzyme Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Triticum aestivum 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Triticum aestivum 4-nitrophenol + octanoate
-
 ?
3.1.1.1 bis(2-ethylhexyl)phthalate + H2O
-
 Triticum aestivum ?
-
 ?
3.1.1.60 4-nitrophenyl acylester + H2O with optimum chain-length C6 to C8, cleavage of bis(2-ethylhexyl)phthalate is 10000times slower than cleavage of 4-nitrophenyl octanoate Triticum aestivum 4-nitrophenol + carboxylic acid
-
 ?
3.1.1.60 bis(2-ethylhexyl)phthalate + H2O
-
 Triticum aestivum 2-ethylhexyl phthalate + 2-ethylhexan-1-ol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 22000, SDS-PAGE Triticum aestivum
3.1.1.60 ? x * 22000, SDS-PAGE Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 DEHP
-
 Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.60 30
-
 assay at Triticum aestivum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.60 0.0000833
-
 bis(2-ethylhexyl)phthalate
-
 Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 6 9
-
 Triticum aestivum
3.1.1.60 6 9 broad Triticum aestivum