EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.106 | Triton X-100 | 0.2% Triton X-100: 4 to 5fold activation, glucosidase I releases in a soluble form by treating microsomal particles with 1.5% Triton X-100, not an absolute requirement for Triton X-100 | Vigna radiata |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.106 | 1-deoxynojirimycin | strong inhibition | Vigna radiata | |
3.2.1.106 | 2,5-Dihydroxymethyl-3,4-dihydroxypyrrolidine | plant pyrrolidine alkaloid, DMDP, 0.04 mM: 50% inhibition | Vigna radiata | |
3.2.1.106 | castanospermine | plant indolizidine alkaloid, strong inhibition | Vigna radiata | |
3.2.1.106 | chloride | inhibitory above 0.3 M, 0.5 M: 30% inhibition | Vigna radiata | |
3.2.1.106 | Co2+ | quite inhibitory | Vigna radiata | |
3.2.1.106 | Cu2+ | quite inhibitory | Vigna radiata | |
3.2.1.106 | ethanolamine | - |
Vigna radiata | |
3.2.1.106 | glycerol | 2% or higher: 25% or more inhibition | Vigna radiata | |
3.2.1.106 | kojibiose | 0.5 mM: 50% inhibition; alpha-1,2-linked glucose disaccharide | Vigna radiata | |
3.2.1.106 | additional information | no inhibition by alpha-1,3-, alpha-1,4-, and alpha-1,6-linked glucose disaccharides; no inhibition by EDTA; no inhibition by nigerose, maltose, isomaltose at 2 mM; no inhibition by swainsonine, deoxymannojirimycin or 1,4-dideoxy-1,4-imino-D-mannitol | Vigna radiata | |
3.2.1.106 | phosphate | inhibitory above 0.3 M, 0.5 M: 30% inhibition | Vigna radiata | |
3.2.1.106 | sulfate | 0.1-0.2 M: 15-20% inhibition, somewhate more inhibitory at higher concentrations | Vigna radiata | |
3.2.1.106 | tricine | - |
Vigna radiata | |
3.2.1.106 | Tris | 20 mM: 50% inhibition | Vigna radiata | |
3.2.1.106 | Zn2+ | quite inhibitory | Vigna radiata |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.106 | endoplasmic reticulum | - |
Vigna radiata | 5783 | - |
3.2.1.106 | membrane | membrane-bound | Vigna radiata | 16020 | - |
3.2.1.106 | microsome | - |
Vigna radiata | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.106 | additional information | no requirement for divalent cations | Vigna radiata |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.106 | 316000 | - |
gel fitration | Vigna radiata |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.106 | Glc3Man9GlcNAc2 + H2O | Vigna radiata | hydrolyses specifically terminal alpha1,2-linked glucose residue | ? | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2 + H2O | Vigna radiata | specific involvement in the N-linked oligosaccharide-processing pathway | ? | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2 + H2O | Vigna radiata | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2 + H2O | Vigna radiata | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
3.2.1.106 | additional information | Vigna radiata | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? | |
3.2.1.106 | additional information | Vigna radiata | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
3.2.1.106 | additional information | Vigna radiata | first enzyme of processing of N-linked oligosaccharides in plants, plant glucosidase I might be analogous to glucosidase I of animal cells | ? | - |
? | |
3.2.1.106 | additional information | Vigna radiata | involved in the formation of high mannose and complex glycoproteins | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.106 | Vigna radiata | - |
mung bean | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.106 | glycoprotein | with high-mannose oligosaccharides | Vigna radiata |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.106 | - |
Vigna radiata |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.106 | Glc3Man9GlcNAc2-[protein] + H2O = Glc2Man9GlcNAc2-[protein] + beta-D-glucopyranose | glucosidase I is a specific alpha-(1,2)-glucosidase | Vigna radiata |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.106 | seedling | - |
Vigna radiata | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.106 | additional information | - |
- |
Vigna radiata |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.106 | -18°C, 10% glycerol, few weeks, no loss of activity | Vigna radiata |
3.2.1.106 | 0°C, very slight loss of activity | Vigna radiata |
3.2.1.106 | 27°C, 40 h, more than 50% activity retained | Vigna radiata |
3.2.1.106 | 37°C, most of activity lost during storage | Vigna radiata |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.106 | Glc3Man9GlcNAc + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Vigna radiata | D-glucose + Glc2Man9GlcNAc | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Vigna radiata | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Vigna radiata | ? | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2 + H2O | specific involvement in the N-linked oligosaccharide-processing pathway | Vigna radiata | ? | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2 + H2O | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Vigna radiata | ? | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2 + H2O | involved in processing of asparagine-linked oligosaccharides | Vigna radiata | ? | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2-lipid + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Vigna radiata | D-glucose + Glc2Man9GlcNAc2-lipid | - |
? | |
3.2.1.106 | Glc3Man9GlcNAc2-peptide + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Vigna radiata | D-glucose + Glc2Man9GlcNAc2-peptide | - |
? | |
3.2.1.106 | additional information | Glc1Man9GlcNAc2: not a substrate | Vigna radiata | ? | - |
? | |
3.2.1.106 | additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Vigna radiata | ? | - |
? | |
3.2.1.106 | additional information | p-nitrophenyl-alpha-D-glucopyranoside: not a substrate | Vigna radiata | ? | - |
? | |
3.2.1.106 | additional information | Glc2Man9GlcNAc2: not a substrate | Vigna radiata | ? | - |
? | |
3.2.1.106 | additional information | substrate and product specificity | Vigna radiata | ? | - |
? | |
3.2.1.106 | additional information | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Vigna radiata | ? | - |
? | |
3.2.1.106 | additional information | involved in processing of asparagine-linked oligosaccharides | Vigna radiata | ? | - |
? | |
3.2.1.106 | additional information | first enzyme of processing of N-linked oligosaccharides in plants, plant glucosidase I might be analogous to glucosidase I of animal cells | Vigna radiata | ? | - |
? | |
3.2.1.106 | additional information | involved in the formation of high mannose and complex glycoproteins | Vigna radiata | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.106 | 27 | - |
40 h: 50% activity retained | Vigna radiata |
3.2.1.106 | 37 | - |
most activity lost during storage | Vigna radiata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.106 | 6.3 | 6.5 | - |
Vigna radiata |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.106 | 5.5 | 6.8 | activity greatly decreases at pH values below 5.5 or above 6.8 | Vigna radiata |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.106 | 6.5 | 7 | most stable at | Vigna radiata |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.106 | 0.04 | - |
2,5-Dihydroxymethyl-3,4-dihydroxypyrrolidine | plant pyrrolidine alkaloid, DMDP | Vigna radiata | |
3.2.1.106 | 0.5 | - |
kojibiose | - |
Vigna radiata |