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Literature summary extracted from

  • Rush, J.S.; Waechter, C.J.
    Topological studies on the enzymes catalyzing the biosynthesis of Glc-P-dolichol and the triglucosyl cap of Glc3Man9GlcNAc2-P-P-dolichol in microsomal vesicles from pig brain: use of the processing glucosidases I/II as latency markers (1998), Glycobiology, 8, 1207-1213.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.106 Triton X-100 activation, maximal activity in the presence of 5-10 mg/ml Triton X-100 Rattus norvegicus
3.2.1.106 Triton X-100 activation, maximal activity in the presence of 0.5-2 mg/ml Triton X-100 Sus scrofa

Application

EC Number Application Comment Organism
3.2.1.106 analysis using the processing glucosidase activity as latency marker for topological studies with microsomal vesicles from nongluconeogenic tissues in addition to brain and cultured mammalian cells lacking glucose 6-phosphate phosphatase, to assess the intactness of microsomal vesicles Rattus norvegicus
3.2.1.106 analysis using the processing glucosidase activity as latency marker for topological studies with microsomal vesicles from nongluconeogenic tissues in addition to brain and cultured mammalian cells lacking glucose 6-phosphate phosphatase, to assess the intactness of microsomal vesicles Sus scrofa

General Stability

EC Number General Stability Organism
3.2.1.106 resistant to trypsin or subtilisin in sealed microsomal vesicles, in unsealed vesicles by the addition of 2 mg/ml Triton X-100, inactivation within 5 min Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.106 1-deoxynojirimycin
-
 Rattus norvegicus
3.2.1.106 1-deoxynojirimycin 0.01 mM: 50% inhibition Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.106 endoplasmic reticulum endoplasmic reticulum vesicles Rattus norvegicus 5783
-
3.2.1.106 endoplasmic reticulum endoplasmic reticulum vesicles Sus scrofa 5783
-
3.2.1.106 endoplasmic reticulum catalytic site/active centre located at lumenal side of endoplasmic reticulum Rattus norvegicus 5783
-
3.2.1.106 endoplasmic reticulum catalytic site/active centre located at lumenal side of endoplasmic reticulum Sus scrofa 5783
-
3.2.1.106 membrane membrane-bound Rattus norvegicus 16020
-
3.2.1.106 membrane membrane-bound Sus scrofa 16020
-
3.2.1.106 microsome microsomal vesicles Rattus norvegicus
-
-
3.2.1.106 microsome microsomal vesicles Sus scrofa
-
-
3.2.1.106 additional information topological studies Rattus norvegicus
-
-
3.2.1.106 additional information topological studies Sus scrofa
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O Rattus norvegicus specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Sus scrofa specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Rattus norvegicus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Rattus norvegicus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
3.2.1.106 additional information Rattus norvegicus involved in processing of asparagine-linked oligosaccharides ?
-
 ?
3.2.1.106 additional information Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Rattus norvegicus
-
-
-
3.2.1.106 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.106 brain
-
 Sus scrofa
-
3.2.1.106 kidney
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O
-
 Rattus norvegicus D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O
-
 Sus scrofa D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Rattus norvegicus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Sus scrofa ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Rattus norvegicus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Rattus norvegicus ?
-
 ?
3.2.1.106 additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Rattus norvegicus ?
-
 ?
3.2.1.106 additional information involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.106 0.01
-
 1-deoxynojirimycin
-
 Sus scrofa