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Literature summary extracted from

  • Herscovics, A.
    Processing glycosidases of Saccharomyces cerevisiae (1999), Biochim. Biophys. Acta, 1426, 275-285.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.106 Triton X-100 about 45% activation of membrane-bound activity, maximum stimulation of membrane-bound activity at 0.5% Triton X-100, no activation of soluble activity Saccharomyces cerevisiae

Application

EC Number Application Comment Organism
3.2.1.106 analysis contributes to knowledge of N-glycan biosynthesis Saccharomyces cerevisiae
3.2.1.106 pharmacology glucosidase I may be a target for antiviral and antitumor drugs, glucosidase I inhibitors are potentially useful as antiviral, antitumor and antimetastatic agents Mammalia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.106 CWH41 gene, encodes yeast glucosidase I Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.106 1-deoxynojirimycin strong inhibition Mammalia
3.2.1.106 1-deoxynojirimycin strong inhibition Saccharomyces cerevisiae
3.2.1.106 castanospermine
-
 Mammalia
3.2.1.106 castanospermine
-
 Saccharomyces cerevisiae
3.2.1.106 kojibiose
-
 Saccharomyces cerevisiae
3.2.1.106 Tris
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.106 endoplasmic reticulum endoplasmic reticulum-resident glycoprotein Mammalia 5783
-
3.2.1.106 endoplasmic reticulum endoplasmic reticulum-resident glycoprotein Saccharomyces cerevisiae 5783
-
3.2.1.106 membrane type-II transmembrane protein Mammalia 16020
-
3.2.1.106 membrane type-II transmembrane protein Saccharomyces cerevisiae 16020
-
3.2.1.106 additional information X-2180, equally distributed between particulate and supernatant fractions Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.106 additional information no requirement for divalent cations Mammalia
3.2.1.106 additional information no requirement for divalent cations Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.106 95000
-
 proteolytically generated soluble form of the enzyme, SDS-PAGE Saccharomyces cerevisiae
3.2.1.106 107000
-
 cloned CWH41 gene encodes a type-II transmembrane protein, Cwh41p, of 107 kDa, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.106 Glc3Man9GlcNAc2 + H2O Mammalia hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O Saccharomyces cerevisiae hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
3.2.1.106 additional information Saccharomyces cerevisiae indirect role in cell wall beta1,6-glucan biosynthesis and/or in beta1,6-glucan insertion into the cell wall ?
-
 ?
3.2.1.106 additional information Mammalia trimming of the glucose residues is identical in Saccharomyces cerevisiae and in mammalian cells ?
-
 ?
3.2.1.106 additional information Saccharomyces cerevisiae trimming of the glucose residues is identical in Saccharomyces cerevisiae and in mammalian cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Mammalia
-
-
-
3.2.1.106 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.106 glycoprotein
-
 Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.106 partial Saccharomyces cerevisiae
3.2.1.106 partial, soluble glucosidase I Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.106 alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 + H2O synthetic trisaccharide Saccharomyces cerevisiae beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Mammalia D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Saccharomyces cerevisiae D-glucose + Glc2Man9GlcNAc2
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Mammalia ?
-
 ?
3.2.1.106 Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information Glc1Man9GlcNAc2: not a substrate Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Mammalia ?
-
 ?
3.2.1.106 additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information Glc2Man9GlcNAc2: not a substrate Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information indirect role in cell wall beta1,6-glucan biosynthesis and/or in beta1,6-glucan insertion into the cell wall Saccharomyces cerevisiae ?
-
 ?
3.2.1.106 additional information trimming of the glucose residues is identical in Saccharomyces cerevisiae and in mammalian cells Mammalia ?
-
 ?
3.2.1.106 additional information trimming of the glucose residues is identical in Saccharomyces cerevisiae and in mammalian cells Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.106 ? x * 95000, SDS-PAGE, reducing conditions Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.2.1.106 CWH41
-
 Saccharomyces cerevisiae
3.2.1.106 Cwh41p calcofluor white hypersensitivity Saccharomyces cerevisiae
3.2.1.106 Cwh41p CWH41 gene encodes a type-II transmembrane protein, Cwh41p Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.106 6.8
-
-
 Saccharomyces cerevisiae